Determination of the melting temperature, heat of fusion, and purity analysis of different samples of zidovudine (AZT) using DSC

Authors

  • Adriano Antunes Souza Araújo Federal University of Sergipe; Departament of Physiology
  • Marília dos Santos Bezerra Federal University of Sergipe; Departament of Physiology
  • Sílvia Storpirtis University of São Paulo; Faculty of Pharmaceutical Sciences; Departament of Pharmacy
  • Jivaldo do Rosário Matos University of São Paulo; Institute of Chemistry; Departament of Fundamental Chemistry

DOI:

https://doi.org/10.1590/S1984-82502010000100005

Keywords:

Zidovudine^i1^spurity determinat, Differential scanning calorimetry, Thermal analysis, Medicines^i1^squality cont, Medicines^i1^squalitative analy

Abstract

The determination of chemical purity, melting range, and variation of enthalpy in the process of characterizing medicines is one of the principal requirements evaluated in quality control of the pharmaceutical industry. In this study, the method of purity determination using DSC was outlined, as well as the application of this technique for the evaluation of commercial samples of zidovudine (AZT) (raw material) supplied by different laboratories. To this end, samples from six different laboratories (A, B, C, D, E, and F) and the standard reference (R) from the United States Pharmacopeia (USP) were analyzed. The DSC curves were obtained in the temperature range of 25 to 200 ºC under the dynamic atmosphere of N2 (50 mL min-1), heating rate of β=2 ºC min-1, using an Al capsule containing approximately 2 mg of sample material. The results demonstrated that the standard reference presented a proportion of 99.83% whereas the AZT samples presented a variation ranging from 97.59 to 99.54%. In addition, the standard reference was found to present a temperature of onset of melting point of 122.80 °C. Regarding the samples of active agents provided by the different laboratories, a variation ranging from 118.70 to 122.87 °C was measured. In terms of ΔHm, the samples presented an average value of 31.12 kJ mol-1.

Downloads

Download data is not yet available.

Downloads

Published

2010-03-01

Issue

Section

Original Papers

How to Cite

Determination of the melting temperature, heat of fusion, and purity analysis of different samples of zidovudine (AZT) using DSC . (2010). Brazilian Journal of Pharmaceutical Sciences, 46(1), 37-43. https://doi.org/10.1590/S1984-82502010000100005