Salivary calcium and phosphate stability in different time and temperature storage

Authors

  • Filipe Ivan Daniel Federal University of Santa Catarina; Health Science Center; Department of Pathology
  • Luiza Lima Federal University of Santa Catarina; Health Science Center; Department of Dentistry
  • Claudia Regina dos Santos Federal University of Santa Catarina; Health Science Center; Department of Pathology

DOI:

https://doi.org/10.1590/s1984-82502016000400011

Abstract

The non-invasive collection and inexpensive nature of saliva has made it an attractive sample for use for diagnosis and research on several diseases. Storage circumstances may affect salivary component concentrations. The objective was to analyze calcium and phosphate stability in saliva samples stored at different conditions. Saliva of healthy people was stored and analyzed by spectrophotometry under different time and temperature conditions in order to evaluate calcium and phosphate stability. Calcium concentration was measured by Arsenazo III reaction at 600nm and phosphate by an acid-molybdate method at 650nm. Using Lin's concordance correlation coefficient (k), we observed very good agreement (k>;0.8) for all samples frozen at -20 oC up to 50 days. Thaw/refreezing cycles can compromise phosphate stability even though there is good agreement (0.61

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Published

2016-12-01

Issue

Section

Articles

How to Cite

Salivary calcium and phosphate stability in different time and temperature storage . (2016). Brazilian Journal of Pharmaceutical Sciences, 52(4), 679-684. https://doi.org/10.1590/s1984-82502016000400011