Grandisin induces apoptosis in leukemic K562 cells

Authors

  • Alane Pereira Cortez Federal University of Goiás; Faculty of Pharmacy; Laboratory of Celullar Toxicology and Pharmacology - FarmaTec
  • Elizabeth Gomes Paulino Menezes Federal University of Goiás; Faculty of Pharmacy; Laboratory of Celullar Toxicology and Pharmacology - FarmaTec
  • Polyana Lopes Benfica Federal University of Goiás; Faculty of Pharmacy; Laboratory of Celullar Toxicology and Pharmacology - FarmaTec
  • Alexandre Pereira dos Santos Federal University of Goiás; Faculty of Pharmacy; Laboratory of Celullar Toxicology and Pharmacology - FarmaTec
  • Larissa Moreira Cleres Federal University of Goiás; Faculty of Pharmacy; Laboratory of Celullar Toxicology and Pharmacology - FarmaTec
  • Higor de Oliveira Ribeiro Federal University of Goiás; Faculty of Pharmacy; Laboratory of Celullar Toxicology and Pharmacology - FarmaTec
  • Eliana Martins Lima Federal University of Goiás; Faculty of Pharmacy; Medicinal Pharmaceutical Chemistry Laboratory
  • Massuo Jorge Kato University of São Paulo; Institute of Chemistry; Chemistry Laboratory of Natural Products
  • Marize Campos Valadares Federal University of Goiás; Faculty of Pharmacy; Laboratory of Celullar Toxicology and Pharmacology - FarmaTec

DOI:

https://doi.org/10.1590/s2175-97902017000115210

Keywords:

Grandisin/antileukemic activities, Leukemic, K562, Apoptosis.

Abstract

In this study, the potential antileukemic activity of grandisin, a lignan extracted from Piper solmsianum, was evaluated against the leukemic line K562. The cytotoxicity of grandisin (0.018 to 2.365 µM) was evaluated in K562 and normal peripheral blood lymphocytes by Trypan Blue Exclusion and MTT methods after 48h exposure to the drug. In both methods, cellular viability was concentration-dependent and the IC50 values were lower than 0.85µM. Analysis of K562 cells after treatment with grandisin showed that the cell cycle was arrested in the G1 phase with a 12.31% increase, while both S and G2 phases decreased. Morphological studies conducted after the exposure of K562 to grandisin revealed changes consistent with the apoptosis process, which was confirmed by anexin V stain and caspase activation. Thus, lignan grandisin showed antileukemic activities against the K562 cell line and the cell death process occurred via apoptosis.

Downloads

Download data is not yet available.

Downloads

Published

2017-01-01

Issue

Section

Articles

How to Cite

Grandisin induces apoptosis in leukemic K562 cells. (2017). Brazilian Journal of Pharmaceutical Sciences, 53(1), e15210-. https://doi.org/10.1590/s2175-97902017000115210