Simultaneous determination of atorvastatin and ezetimibe from combined pharmaceutical products by micellar electrokinetic capillary chromatography

Authors

  • Blanka Székely-Szentmiklósi University of Medicine and Pharmacy; Faculty of Pharmacy; Department of Pharmaceutical Chemistry
  • Gabriel Hancu University of Medicine and Pharmacy; Faculty of Pharmacy; Department of Pharmaceutical Chemistry
  • István Székely-Szentmiklósi Gedeon Richter Romania; Technological Development Department
  • Béla Kovács Gedeon Richter Romania; Technological Development Department
  • Hajnal Kelemen University of Medicine and Pharmacy; Faculty of Pharmacy; Department of Pharmaceutical Chemistry

DOI:

https://doi.org/10.1590/s2175-97902017000116122

Keywords:

Micellar Electrokinetic Capillary Chromatography, Fixed Dose Drug Combination, Atorvastatin, Ezetimibe.

Abstract

A rapid and sensitive micellar electrokinetic capillary chromatography method with UV photodiode-array detection was developed for the simultaneous determination of atorvastatin and ezetimibe in fixed dose drug combination. Experimental conditions such as buffer concentration and pH, surfactant concentration, system temperature, applied voltage, injection parameters were optimized in order to improve the efficiency of the separation. The best results were obtained when using fused silica capillary (48 cm length X 50 µm ID) and 25 mM borate buffer electrolyte at pH 9.3 containing 25 mM SDS, + 30 kV applied voltage, 20 ºC system temperature. The separation was achieved in approximately 2 minutes, with a resolution of 7.02, the order of migration being atorvastatin followed by ezetimibe. The analytical performance of the method was verified with regard to linearity, precision, robustness and the limit of detection and quantification were calculated.

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Published

2017-01-01

Issue

Section

Articles

How to Cite

Simultaneous determination of atorvastatin and ezetimibe from combined pharmaceutical products by micellar electrokinetic capillary chromatography. (2017). Brazilian Journal of Pharmaceutical Sciences, 53(1), e16122-. https://doi.org/10.1590/s2175-97902017000116122