Adhesion and biofilm formation by Staphylococcus aureus from food processing plants as affected by growth medium, surface type and incubation temperature

Authors

  • Heloísa Maria Ângelo Jerônimo Federal University of Paraíba; Health Sciences Center; Department of Nutrition; Laboratory of Food Microbiology
  • Rita de Cássia Ramos do Egypto Queiroga Federal University of Paraíba; Health Sciences Center; Department of Nutrition; Laboratory of Food Microbiology
  • Ana Caroliny Vieira da Costa Federal University of Paraíba; Health Sciences Center; Department of Nutrition; Laboratory of Food Microbiology
  • Isabella de Medeiros Barbosa Federal University of Paraíba; Health Sciences Center; Department of Nutrition; Laboratory of Food Microbiology
  • Maria Lúcia da Conceição Federal University of Paraíba; Health Sciences Center; Department of Nutrition; Laboratory of Food Microbiology
  • Evandro Leite de Souza Federal University of Paraíba; Health Sciences Center; Department of Nutrition; Laboratory of Food Microbiology

DOI:

https://doi.org/10.1590/S1984-82502012000400018

Keywords:

Staphylococcus aureus^i1^sfood process, Biofilm, Surfaces, Sanitizers^i1^seffic

Abstract

This study assessed the effect of different growth media [BHI broth, BHI broth plus glucose (10 g/100 mL) and BHI broth plus NaCl (5 g/100 mL)] and incubation temperatures (28 or 37 ºC) on the adherence, detachment and biofilm formation on polypropylene and stainless steel surfaces (2 x 2 cm coupons) for a prolonged period (24-72 h) by some strains of Staphylococcus aureus (S3, S28 and S54) from food processing plants. The efficacy of the sanitizers sodium hypochlorite (250 mg/mL) and peracetic acid (30 mg/mL) in reducing the number of viable bacterial cells in a preformed biofilm was also evaluated. S. aureus strains adhered in highest numbers in BHI broth, regardless of the type of surface or incubation temperature. Cell detachment from surfaces revealed high persistence over the incubation period. The number of cells needed for biofilm formation was noted in all experimental systems after 3 days. Peracetic acid and sodium hypochlorite were not efficient in completely removing the cells of S. aureus adhered onto polypropylene and stainless steel surfaces. From these results, the assayed strains revealed high capacities to adhere and form biofilms on polypropylene and stainless steel surfaces under the different growth conditions, and the cells in biofilm matrixes were resistant to total removal when exposed to the sanitizers sodium hypochlorite and peracetic acid.

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Published

2012-12-01

Issue

Section

Articles

How to Cite

Adhesion and biofilm formation by Staphylococcus aureus from food processing plants as affected by growth medium, surface type and incubation temperature . (2012). Brazilian Journal of Pharmaceutical Sciences, 48(4), 737-745. https://doi.org/10.1590/S1984-82502012000400018