Anti-inflammatory and healing action of oral gel containing borneol monoterpene in chemotherapy-induced mucositis in rats ( Rattus norvegicus )

Authors

  • Braz José do Nascimento-Júnior Federal University of São Francisco Valley; Faculty of Pharmacy; Department of Pharmacy
  • Lucas de Souza Brito Federal University of São Francisco Valley; Faculty of Medicine; Department of Pathology
  • Walquíria Nunes Barros Federal University of São Francisco Valley; Faculty of Pharmacy; Department of Pharmacy
  • Daniela Marques Gonçalves Federal University of São Francisco Valley; Faculty of Pharmacy; Department of Pharmacy
  • Luana de Souza Matos Federal University of São Francisco Valley; Faculty of Pharmacy; Department of Pharmacy
  • Cínthia Reyjane Borges Nascimento Federal University of São Francisco Valley; Faculty of Pharmacy; Department of Pharmacy
  • Luciano Augusto de Araújo Ribeiro Federal University of São Francisco Valley; Faculty of Pharmacy; Department of Pharmacy
  • Ricardo Santana de Lima Federal University of São Francisco Valley; Faculty of Medicine; Department of Pathology
  • René Geraldo Cordeiro Silva-Júnior Federal University of São Francisco Valley; Faculty of Veterinary Medicine; Department of Experimental Practice
  • Sílvio Alan Gonçalves Bomfim Reis Federal University of São Francisco Valley; Faculty of Pharmacy; Department of Pharmacy
  • Talita Mota Gonçalves Federal University of São Francisco Valley; Faculty of Medicine; Department of Pathology
  • Elba Lúcia Cavalcanti de Amorim Federal University of Pernambuco; Faculty of Pharmacy; Department of Pharmacy

DOI:

https://doi.org/10.1590/s2175-97902017000300081

Keywords:

Chemotherapy, Oral gel/Borneol monoterpene/effects, Oral gel/Borneol monoterpene/action anti-inflammatory, Oral gel/Borneol monoterpene/healing action, Bornyl alcohol/effects, Palliative care

Abstract

The aim of this study was to investigate the effect of gels containing the monoterpene borneol in induced oral mucositis using an animal model. Gels were prepared with borneol at 1.2% and 2.4% (w/w). Oral mucositis was induced by administration of three doses of 5-fluorouracil (30 mg/kg, i.p.) and injury with acetic acid (50%, v/v) soaked in filter paper applied to right cheek mucosa for 60s. Four subgroups comprising 12 animals each were formed. Six animals from each group were sacrificed at days seven and fourteen after oral mucositis induction. Mucous samples were processed and stained with hematoxylin-eosin and Masson’s Trichrome. The semiquantitative evaluation involved observation of inflammatory parameters. ImageJ® software was used in the quantitative evaluation. For statistical analyses, Two-way ANOVA, followed by Tukey’s post-test (p <0.05), were employed. Borneol 2.4% gel proved effective in the treatment of oral mucositis with statistically significant differences between groups for angiogenesis control, inflammatory cell count reduction and percentage neoformed collagen increase. The confirmation of anti-inflammatory and healing action of borneol in oral mucositis in rats renders it a good marker for predicting this activity for plant extracts rich in this substance.

Downloads

Download data is not yet available.

Downloads

Published

2017-01-01

Issue

Section

Articles

How to Cite

Anti-inflammatory and healing action of oral gel containing borneol monoterpene in chemotherapy-induced mucositis in rats ( Rattus norvegicus ). (2017). Brazilian Journal of Pharmaceutical Sciences, 53(3), e00081. https://doi.org/10.1590/s2175-97902017000300081