Immunophenotyping of leukocytes in bovine placenta

Authors

  • Thais Martins Chucri Universidade de São Paulo, Faculdade de Medicina Veterinária, Departamento de Cirurgia Centro Universitário das Faculdades Metropolitanas Unidas, Faculdade de Medicina Veterinária Universidade Metropolitana de Santos, Faculdade de Medicina Veterinária, Santos
  • Janaína Monteiro Universidade de São Paulo, Faculdade de Medicina Veterinária, Departamento de Cirurgia
  • Ana Rita de Lima Universidade Federal Rural da Amazônia, Laboratório de Pesquisa Morfológica Animal
  • Paula Andrea de Santis Bastos Centro Universitário das Faculdades Metropolitanas Unidas, Faculdade de Medicina Veterinária Universidade Metropolitana de Santos, Faculdade de Medicina Veterinária
  • Vanessa Aparecida Feijó de Souza Centro Universitário das Faculdades Metropolitanas Unidas, Faculdade de Medicina Veterinária
  • José Roberto Kfoury Júnior Universidade de São Paulo, Faculdade de Medicina Veterinária, Departamento de Cirurgia

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.2017.116338

Keywords:

Placentome, Intercaruncular region, Immunology, Maternal-fetal tolerance

Abstract

Lymphocytes and macrophages are the main white cells involved in fetal-maternal tolerance. Little is known about these leukocytes in bovine placenta, such as the quantity and location of these cells. Thus, the objective of this study was to identify lymphocyte and macrophage populations in bovine placenta using specific markers and flow cytometry. This study analyzed samples of placentomes and intercaruncular regions of cows in the three quarters of pregnancy. In the placentomes, during the first quarter of pregnancy, mean percentage of labeled CD3+ cells was 2.34%; CD8+, 1.28%; CD14+, 1.66%; and CD335+, 0.96%. For the intercaruncular region, percentage of CD3+ cells was 0.71%; CD8+, 1.63%; CD14+, 2.81%; and CD335+, 2.81%. In the second quarter, placentomes showed 0.94% CD3+ cells; 0.77% CD8+; 0.72% CD14+; and 0.51% CD335+. In the intercaruncular region, percentage of labeled CD3+ cells was 0.50%; CD8+, 1.81%; CD14+, 2.64%; and CD335+, 0.51%. In the third quarter, placentomes showed labeling of 0.88% CD3+; 0.66% CD8+; 1.06% CD14+; and 0.74% CD335+ cells. In the intercaruncular region, percentage of labeled CD3+ cells was 0.19%; CD8+, 2.23%; CD14+, 2.43%; and CD335+, 0.16%. The results showed that there was a greater immunomarking of leukocytes CD3+ and CD335+ in the placentome when compared to the intercaruncular region during the third trimester. It can be concluded that leukocytes populations in bovine placenta is reduced, probably because of the syndesmochorial characteristic of bovine placenta. This represents a significant barrier for the immunological system of the mother, sharply decreasing the exposure of the conceptus to the mother’s immune system.

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Author Biographies

  • Thais Martins Chucri, Universidade de São Paulo, Faculdade de Medicina Veterinária, Departamento de Cirurgia Centro Universitário das Faculdades Metropolitanas Unidas, Faculdade de Medicina Veterinária Universidade Metropolitana de Santos, Faculdade de Medicina Veterinária, Santos
    Departamento de Cirurgia
  • Janaína Monteiro, Universidade de São Paulo, Faculdade de Medicina Veterinária, Departamento de Cirurgia
    Departamento de Cirurgia
  • Ana Rita de Lima, Universidade Federal Rural da Amazônia, Laboratório de Pesquisa Morfológica Animal
    Faculdade de Medicina Veterinária
  • Paula Andrea de Santis Bastos, Centro Universitário das Faculdades Metropolitanas Unidas, Faculdade de Medicina Veterinária Universidade Metropolitana de Santos, Faculdade de Medicina Veterinária
    Departamento de Cirurgia
  • Vanessa Aparecida Feijó de Souza, Centro Universitário das Faculdades Metropolitanas Unidas, Faculdade de Medicina Veterinária
    Laboratório de Pesquisa Morfológica Animal

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Published

2017-08-18

Issue

Section

FULL ARTICLE

How to Cite

Immunophenotyping of leukocytes in bovine placenta. (2017). Brazilian Journal of Veterinary Research and Animal Science, 54(2), 129-138. https://doi.org/10.11606/issn.1678-4456.bjvras.2017.116338