Oral mucosa: an alternative epidermic cell source to develop autologous dermal-epidermal substitutes from diabetic subjects

Authors

  • Daniela GUZMÁN-URIBE Universidad Autónoma de San Luis Potosí; Facultad de Medicina
  • Keila Neri ALVARADO-ESTRADA Universidad Autónoma de San Luis Potosí; Facultad de Estomatología
  • Mauricio PIERDANT-PÉREZ Universidad Autónoma de San Luis Potosí; Facultad de Medicina
  • Bertha TORRES-ÁLVAREZ Hospital Central Dr Ignacio Morones Prieto; Departamento de Dermatología
  • Jesus Martin SÁNCHEZ-AGUILAR Universidad Autónoma de San Luis Potosí; Facultad de Medicina
  • Raúl ROSALES-IBÁÑEZ Universidad Nacional Autónoma de México; Facultad de Estudios Superiores Iztacala; Laboratorio en Ingeniería Tisular y Medicina Traslacional

DOI:

https://doi.org/10.1590/1678-77572016-0217

Keywords:

Oral mucosa, Skin substitutes, Biological dressings

Abstract

Oral mucosa has been highlighted as a suitable source of epidermal cells due to its intrinsic characteristics such as its higher proliferation rate and its obtainability. Diabetic ulcers have a worldwide prevalence that is variable (1%-11%), meanwhile treatment of this has been proven ineffective. Tissue-engineered skin plays an important role in wound care focusing on strategies such autologous dermal-epidermal substitutes. Objective The aim of this study was to obtain autologous dermal-epidermal skin substitutes from oral mucosa from diabetic subjects as a first step towards a possible clinical application for cases of diabetic foot. Material and Methods Oral mucosa was obtained from diabetic and healthy subjects (n=20 per group). Epidermal cells were isolated and cultured using autologous fibrin to develop dermal-epidermal in vitro substitutes by the air-liquid technique with autologous human serum as a supplement media. Substitutes were immunocharacterized with collagen IV and cytokeratin 5-14 as specific markers. A Student´s t- test was performed to assess the differences between both groups. Results It was possible to isolate epidermal cells from the oral mucosa of diabetic and healthy subjects and develop autologous dermal-epidermal skin substitutes using autologous serum as a supplement. Differences in the expression of specific markers were observed and the cytokeratin 5-14 expression was lower in the diabetic substitutes, and the collagen IV expression was higher in the diabetic substitutes when compared with the healthy group, showing a significant difference. Conclusion Cells from oral mucosa could be an alternative and less invasive source for skin substitutes and wound healing. A difference in collagen production of diabetic cells suggests diabetic substitutes could improve diabetic wound healing. More research is needed to determine the crosstalk between components of these skin substitutes and damaged tissues.

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Published

2017-04-01

Issue

Section

Original Articles

How to Cite

Oral mucosa: an alternative epidermic cell source to develop autologous dermal-epidermal substitutes from diabetic subjects. (2017). Journal of Applied Oral Science, 25(2), 186-195. https://doi.org/10.1590/1678-77572016-0217