18β-glycyrrhetinic acid attenuates global cerebral ischemia/reperfusion-induced cardiac damage in C57BL/J6 mice
DOI:
https://doi.org/10.1590/Keywords:
Global cerebral I/R, 18β-glycyrrhetinic acid, Oxidative stress, Heart injuryAbstract
The aim of the present study is to investigate the cardioprotective effects of 18β-glycyrrhetinic acid (18β -GA) against oxidative and histological damage caused by global cerebral ischemia/ reperfusion (I/R) in C57BL/J6 mice. All male mice (n:40) were randomly divided into four groups: (1) sham-operated (Sham), (2) I/R, (3) 18β-GA, and (4) 18β -GA+I/R. Ischemia was not applied to the sham and 18β-GA groups. In the I/R group, the bilateral carotid arteries were clipped for 15 min to induce ischemia, and the mice were treated with the vehicle for 10 days. In the 18β-GA group, the mice were given 18β-GA (100 mg/kg) for 10 days following a median incision without carotid occlusion. In the 18β-GA+I/R group, the ischemic procedure performed to the I/R model was applied to the animals and afterwards they were intraperitoneally (i.p.) treated with 18β-GA (100 mg/kg) for 10 days. It was found that global cerebral I/R increased TBARS levels and decreased antioxidant parameters. The 18β-GA treatment decreased the level of TBARS and increased GSH, GPx, CAT, SOD activities. Also, the control group cardiac tissue samples were observed to have a normal histological appearance with the Hematoxylin-Eosin staining method. Histopathological damage was observed in the heart tissue samples belonging to the I/R group. The 18β-GA treatment ameliorates oxidative and histological injury in the heart tissue after global ischemia reperfusion, and may be a beneficial alternative treatment.
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Abd El-Twab SM, Hozayen WG, Hussein OE, Mahmoud AM. 18beta-Glycyrrhetinic acid protects against methotrexate-induced kidney injury by up-regulating the Nrf2/ARE/HO-1 pathway and endogenous antioxidants. Ren Fail. 2016;38(9):1516-27.
Aebi H. Catalase in vitro. Methods in enzymol. 1984;105:121-6.
Akai F, Yanagihara T. Identity of the dorsal hippocampal region most vulnerable to cerebral ischemia. Brain Res. 1993;603(1):87-95.
Ambrosio G, Flaherty JT, Duilio C, Tritto I, Santoro G, Elia PP, et al. Oxygen radicals generated at reflow induce peroxidation of membrane lipids in reperfused hearts. J Clin Investig. 1991;87(6):2056-66.
Aslı MN, Hosseinzadeh H. Review of pharmacological effects of Glycyrrhiza sp. and its bioactive compounds. Phytother Res. 2008;22(6):709-24.
Burcu GB, Osman C, Aslı C, Namik OM, Nese BT. The protective cardiac effects of Β-myrcene after global cerebral ıschemia/reperfusion in C57BL/J6 mouse. Acta Cir Bras. 2016;31(7):456-62.
Ciftci O, Oztanir MN, Cetin A. Neuroprotective effects of β-myrcene following global cerebral ischemia/reperfusion- mediated oxidative and neuronal damage in a C57BL/J6 mouse. Neurochem Res. 2014;39(9):1717-23.
Dai LY, Cheng BH, Li J. Effect of curcumin on cerebral ischemia-reperfusion injury in rats. Zhong Yao Cai. 2015;38(2):344-49.
Danton GH, Dietrich WD. Inflammatory mechanisms after ischemia and stroke. J Neuropathol Exp Neurol. 2003;62(2):127-36.
Durak MA, Öztanir MN, Türkmen NB, Ciftci O, Taşlıdere A, Tecellioğlu M, et al. Chrysin prevents brain damage caused by global cerebral ischemia/reperfusion in a C57BL/ J6 mouse model. Turk J Med Sci. 2016;46(6):1926-33.
Kalaiarasi P, Pugalendi KV. Protective effect of 18b-glycyrrhetinic acid on lipid peroxidation and antioxidant enzymes in experimental diabetes. J Pharm Res. 2011;4(1):107-11.
Karmazyn M. Ischemic and reperfusion injury in the heart: cellular mechanisms and pharmacological intervention. Can J Physiol Pharmacol. 1991;69(6):719-30.
Kho AR, Choi BY, Lee SH, Hong DK, Lee SH, Jeong JH, et al. Effects of protocatechuic acid (PCA) on global cerebral ischemia-induced hippocampal neuronal death. Int J Mol Sci. 2018;19(5):1420.
Leak RK, Li P, Zhang F, Sulaiman HH, Weng Z, Wang G, et al. Apurinic/apyrimidinic endonuclease 1 upregulation reduces oxidative DNA damage and protects hippocampal neurons from ischemic injury. Antioxid Redox Signal. 2015;22(2):135-48.
Lejay A, Fang F, John R, Van JA, Barr M, Thaveau F, et al. Ischemia reperfusion injury, ischemic conditioning and diabetes mellitus. J Mol Cell Cardiol. 2016;91:11-22.
Llinas R, Barbut D, Caplan LR. Neurologic complications of cardiac surgery. Prog Cardiovasc Dis. 2000;43(2):101-12.
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with folin phenol reagent. J Biol Chem. 1951;193(1):265-75.
Mahmoud AM, Al Dera HS. 18β-Glycyrrhetinic acid exerts protective effects against cyclophosphamide- induced hepatotoxicity: potential role of PPARγ and Nrf2 upregulation. Genes Nutr. 2015;10(6):1-13.
Nikonenko AG, Radenovic L, Andjus PR, Skibo GG. Structural features of ischemic damage in the hippocampus. Anat Rec. 2009;292(12):1914-21.
Nishikata R, Kato N, Hiraiwa K. Oxidative stress may be involved in distant organ failure in tourniquet shock model mice. Leg Med. 2014;16(2):70-5.
Nita DA, Nita V, Spulber S, Moldovan M, Popa DP, Zagrean AM, et al. Oxidative damage following cerebral ischemia dependson reperfusion: a biochemical study in rat. J Cell Mol Med. 2001;5(2):163-70.
Ogawa SS, Koga K, Kuwabara J, Brett B, Morrow SA, Morris JP, et al. Hypoxia-induced increased permeability of endothelial monolayers occurs through lowering of cellular cAMP levels. Am J Physiol. 1992;262(3 Pt 1):546-54.
Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med. 1967;70(1):158-69.
Park SW, Kim M, Brown KM, D’Agati VD, Lee HT. Paneth cell-derived interleukin-17A causes multiorgan dysfunction after hepatic ischemia and reperfusion injury. Hepatology. 2011;53(5):1662-75.
Patel RP, Lang JD, Smith AB, Crawford JH. Redox therapeutics in hepatic ischemia reperfusion injury. World J Hepatol. 2014;6(1):1-8.
Pinelli A, Trivulzio S, Brenna S, Rossoni G. Plasma cardiac necrosis markers C-troponin I and creatine kinase, associated with increased malondialdehyde levels, induced in rabbits by means of 5-aminolevulinic acid injection. Pharmacology. 2009;84(5):314-21.
Sanderson TH, Reynolds CA, Kumar R, Przyklenk K, Hüttemann M. Molecular mechanisms of ischemia- reperfusion injury in brain: pivotal role of the mitochondrial membrane potential in reactive oxygen species generation. Mol Neurobiol. 2013;47(1):9-23.
Sedlak J, Lindsay RH. Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman’s reagent. Anal Biochem. 1968;25(1):192-205.
Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clin Chem. 1988;34(3):497-500.
Wang ZY, Agarwal R, Zhou ZC, Bickers DR, Mukhtar H. Inhibition of mutagenicity in Salmonella typhimurium and skin tumor initiating and tumor promoting activities in SENCAR mice by glycyrrhetinic acid: comparison of 18 alpha- and 18 beta-stereoisomers. Carcinogenesis. 1991;12(2):187-92.
Yagi K. Simple assay for the level of total lipid peroxides in serum or plasma. Methods Mol Biol. 1998;108:101-6.
Yaman M, Eser O, Cosar M, Bas O, Sahin O, Mollaoglu H, et al. Oral administration of avocado soybean unsaponifiables (ASU) reduces ischemic damage in the rat hippocampus. Arch Med Res. 2007;38(5):489-94.
Yang Y, Liu P, Chen L, Liu Z, Zhang H, Wang J, et al. Therapeutic effect of Ginkgo biloba polysaccharide in rats with focal cerebral ischemia/reperfusion (I/R) injury. Carbohydr Polym. 2013;98(2):1383-88.
Yasuda N, Ishii T, Oyama D, Fukuta T, Agato Y, Sato A, et al. Neuroprotective effect of nobiletin on cerebral ischemia-reperfusion injury in transient middle cerebral arteryoccluded rats. Brain Res . 2014;1559:46-54.
Yonekura I, Kawahara N, Nakatomi H, Furuya K, Kirino T. A model of global cerebral ischemia in C57 BL/6 mice. J Cereb. 2004;24(2):151-8.
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