Effects of sea buckthorn granules on the lungs of rats with chronic bronchitis: a study based on the P38 MAPK/P65 NF-ΚB signaling pathway
DOI:
https://doi.org/10.1590/s2175-97902026e24728Keywords:
Sea Buckthorn Granules, Chronic bronchitis, Network pharmacology, MAPK pathwayAbstract
This study was conducted to explore the mechanism of Sea Buckthorn Granules (SBG) in treating chronic bronchitis (CB) in rats. The primary chemical components of SBG were identified using UPLC-Q-TOF-MS/MS. A rat model of CB was established through smoke inhalation combined with lipopolysaccharide (LPS) stimulation, followed by oral administration of SBG. The serum levels of TNF-α, IL-6, and IL-1β in rats were measured using ELISA. Pathological changes in lung tissues were observed through HE and toluidine blue staining. Furthermore, the main components and pathways involved in the therapeutic effects of SBG on CB were predicted using network pharmacology. In addition, p38 and NF-κBp65 protein expression as well as their phosphorylation levels in rat lung tissues were measured by western blot. Compared to the model group, treatment with SBG significantly alleviated cough symptoms, and Mitigating body weight loss in CB rats (P
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