Cytotoxicity and genotoxicity of Cassia angustifolia decoct

Višnja Madić; Bojana Maksimović; Nemanja Mladenović; Aleksandra Petrović; Nedeljko Manojlović; Marina Jušković; Perica Vasiljević

doi:10.1590/s2175-97902026e24684

Autores

Višnja Madić Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Serbia , University of Nis https://orcid.org/0000-0002-6040-378X
Bojana Maksimović Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Serbia , University of Nis
Nemanja Mladenović Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Serbia , University of Nis
Aleksandra Petrović Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Serbia , University of Nis
Nedeljko Manojlović Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia , University of Kragujevac
Marina Jušković University of Nis , Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Serbia
Perica Vasiljević Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Serbia , University of Nis

DOI:

https://doi.org/10.1590/s2175-97902026e24684

Palavras-chave:

Cassia angustifolia, Decoct, Cyto-toxicology, Geno-toxicology

Resumo

Cassia angustifolia is one of the most commonly used medicinal plants globally. Although many studies evaluated its biological activities, their results led to opposing results, particularly when it comes to the toxicity of this species. Thus, this study aimed to further evaluate the biological properties of Sennae folium decoct especially its cytotoxicity and genotoxicity. HPLC analysis revealed the presence of 8-glucosyl rhein, sennoside A, sennoside B, sennoside C, sennoside D and rhein. DPPH test showed low antioxidant activity. RBCs hemolytic assay, Allium cepa test and lipid peroxidation evaluation confirmed the high cytotoxicity of the tested decoct when used in concentrations of 1500 μg/ml, similar to the one observed in positive controls, cells treated with H₂O₂ . However, the genotoxicity assessment showed that the genotoxic properties of even the high tested concentrations of the Sennae folium decoct were much lower than the ones seen in the positive controls (p < 0.001). It can be concluded that the toxicity of the higher concentrations of this Cassia species decoct is mostly caused by its actions on the cell membrane level.

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Cytotoxicity and genotoxicity of Cassia angustifolia decoct

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