Increased whitening efficacy and reduced cytotoxicity are achieved by the chemical activation of a highly concentrated hydrogen peroxide bleaching gel

Authors

  • Diana Gabriela Soares Universidade de São Paulo, Faculdade de Odontologia de Bauru, Departamento de Dentística, Endodontia e Materiais Odontológicos, Bauru, São Paulo http://orcid.org/0000-0002-1485-6104
  • Natália Marcomini Universidade Estadual Paulista – UNESP, Faculdade de Odontologia de Araraquara, Departamento de Dentística Restauradora, Araraquara, São Paulo http://orcid.org/0000-0002-9274-3623
  • Carla Caroline de Oliveira Duque Universidade Estadual Paulista – UNESP, Faculdade de Odontologia de Araraquara, Departamento de Materiais Dentários e Prótese, Araraquara, São Paulo http://orcid.org/0000-0002-5898-4132
  • Ester Alves Ferreira Bordini Universidade Estadual Paulista – UNESP, Faculdade de Odontologia de Araraquara, Departamento de Materiais Dentários e Prótese, Araraquara, São Paulo http://orcid.org/0000-0002-4178-5794
  • Uxua Ortecho Zuta Universidade Estadual Paulista – UNESP, Faculdade de Odontologia de Araraquara, Departamento de Materiais Dentários e Prótese, Araraquara, São Paulo http://orcid.org/0000-0002-2922-3609
  • Fernanda Gonçalves Basso Universidade Estadual Paulista – UNESP, Faculdade de Odontologia de Araraquara, Departamento de Fisiologia e Patologia, Araraquara, São Paulo http://orcid.org/0000-0002-7170-2371
  • Josimeri Hebling Universidade Estadual Paulista – UNESP, Faculdade de Odontologia de Araraquara, Departamento de Clínica Infantil, Araraquara, São Paulo http://orcid.org/0000-0002-2846-2325
  • Carlos Alberto de Souza Costa Universidade Estadual Paulista – UNESP, Faculdade de Odontologia de Araraquara, Departamento de Fisiologia e Patologia, Araraquara, São Paulo https://orcid.org/0000-0002-7455-6867

DOI:

https://doi.org/10.1590/

Keywords:

Tooth bleaching, Dental pulp, Cytotoxicity, Odontoblasts

Abstract

Objective: This study was designed for the chemical activation of a 35% hydrogen peroxide (H2O2) bleaching gel to increase its whitening effectiveness and reduce its toxicity. Methodology: First, the bleaching gel - associated or not with ferrous sulfate (FS), manganese chloride (MC), peroxidase (PR), or catalase (CT) - was applied (3x 15 min) to enamel/dentin discs adapted to artificial pulp chambers. Then, odontoblast-like MDPC-23 cells were exposed for 1 h to the extracts (culture medium + components released from the product), for the assessment of viability (MTT assay) and oxidative stress (H2DCFDA). Residual H2O2 and bleaching effectiveness (DE) were also evaluated. Data were analyzed with one-way ANOVA complemented with Tukey’s test (n=8. p<0.05). Results:
All chemically activated groups minimized MDPC-23 oxidative stress generation; however, significantly higher cell viability was detected for MC, PR, and CT than for plain 35% H2O2 gel. Nevertheless, FS, MC, PR, and CT reduced the amount of residual H2O2 and increased bleaching effectiveness. Conclusion:
Chemical activation of 35% H2O2 gel with MC, PR, and CT minimized residual H2O2 and pulp cell toxicity; but PR duplicated the whitening potential of the bleaching gel after a single 45-minute session.

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Published

2024-09-04

Issue

Vol. 27 (2019)

Section

Original Articles

License

Copyright (c) 2024 Diana Gabriela Soares, Natália Marcomini, Carla Caroline de Oliveira Duque, Ester Alves Ferreira Bordini, Uxua Ortecho Zuta, Fernanda Gonçalves Basso, Josimeri Hebling, Carlos Alberto de Souza Costa

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Todo o conteúdo do periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons do tipo atribuição CC-BY.

 

 

How to Cite

Soares, D. G., Marcomini, N., Duque, C. C. de O., Bordini, E. A. F., Zuta, U. O., Basso, F. G., Hebling, J., & Costa, C. A. de S. (2024). Increased whitening efficacy and reduced cytotoxicity are achieved by the chemical activation of a highly concentrated hydrogen peroxide bleaching gel. Journal of Applied Oral Science, 27, e20180453. https://doi.org/10.1590/