Effects of CEACAM1 in oral keratinocytes on HO-1 expression induced by Candida β-glucan particles

Authors

  • Miyuki Sakuma Hiroshima University, Graduate School of Biomedical and Health Sciences, Department of Oral and Maxillofacial Surgery, Minami-Ku, Hiroshima
  • Kouji Ohta Hiroshima University, Graduate School of Biomedical and Health Sciences, Program of Oral Health Sciences, Department of Public Oral Health, Minami-Ku, Hiroshima http://orcid.org/0000-0002-0603-9171
  • Shohei Fukada Hiroshima University, Graduate School of Biomedical and Health Sciences, Department of Oral and Maxillofacial Surgery, Minami-Ku, Hiroshima
  • Misaki Akagi Hiroshima University, Graduate School of Biomedical and Health Sciences, Department of Oral and Maxillofacial Surgery, Minami-Ku, Hiroshima
  • Hiroki Kato Hiroshima University, Graduate School of Biomedical and Health Sciences, Department of Oral and Maxillofacial Surgery, Minami-Ku, Hiroshima
  • Yoko Ishida Hiroshima University, Graduate School of Biomedical and Health Sciences, Department of Oral and Maxillofacial Surgery, Minami-Ku, Hiroshima
  • Takako Naruse Hiroshima University, Graduate School of Biomedical and Health Sciences, Department of Oral and Maxillofacial Surgery, Minami-Ku, Hiroshima
  • Masaaki Takechi National Hospital Organization Kure Medical Center and Chugoku Cancer Center, Department of Dentistry, Oral and Maxillofacial Surgery, Kure
  • Hideo Shigeishi Hiroshima University, Graduate School of Biomedical and Health Sciences, Program of Oral Health Sciences, Department of Public Oral Health, Minami-Ku, Hiroshima http://orcid.org/0000-0003-0883-4299
  • Hiromi Nishi Hiroshima University Hospital, Department of General Dentistry, Minami-Ku, Hiroshima
  • Tomonao Aikawa Hiroshima University, Graduate School of Biomedical and Health Sciences, Department of Oral and Maxillofacial Surgery, Minami-Ku, Hiroshima

DOI:

https://doi.org/10.1590/1678-7757-2022-0158%20

Keywords:

Candida albicans, CEACAM1, β-glucan-containing particle, HO-1, Oral keratinocytes

Abstract

Objective: Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) is a member of the carcinoembryonic antigen family. Although its expression has been found in chronic oral inflammatory epithelium, this study aimed to know whether CEACAM1 in oral keratinocytes participates in host immune response against Candida albicans . Methodology: We investigated CEACAM1 expression in oral keratinocytes induced by C. albicans as well as by Candida cell wall component β-glucan particles (β-GPs). Furthermore, the effects of CEACAM1 on β-GPs-induced heme oxygenase-1 (HO-1) expression and its related signals were examined. Results: Fluorescence staining showed CEACAM1 expression in oral keratinocytes (RT7) cells, whereas quantitative reverse transcription (RT)-PCR indicated that both live and heat-killed C. albicans increased CEACAM1 mRNA expression in RT7 cells. Examinations using quantitative RT-PCR and western blotting indicated that CEACAM1 expression was also increased by β-GPs derived from C. albicans . Specific siRNA for CEACAM1 decreased HO-1 expression induced by β-GPs from C. albicans as well as the budding yeast microorganism Saccharomyces cerevisiae . Moreover, knockdown of CEACAM1 decreased β-GPs-induced ROS activity in the early phase and translocation of Nrf2 into the nucleus. Conclusion: CEACAM1 in oral keratinocytes may have a critical role in regulation of HO-1 for host immune defense during Candida infection.

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Published

2022-11-10

Issue

Vol. 30 (2022)

Section

Original Articles

License

Copyright (c) 2022 Journal of Applied Oral Science

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Todo o conteúdo do periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons do tipo atribuição CC-BY.

 

 

How to Cite

Sakuma, M., Ohta, K., Fukada, S., Akagi, M., Kato, H., Ishida, Y. ., Naruse, T., Takechi, M., Shigeishi, H., Nishi, H., & Aikawa, T. . (2022). Effects of CEACAM1 in oral keratinocytes on HO-1 expression induced by Candida β-glucan particles. Journal of Applied Oral Science, 30, e20220158. https://doi.org/10.1590/1678-7757-2022-0158