Effects of Thai propolis mixed in mineral trioxide aggregate on matrix metalloproteinase-2 expression and activity in inflamed human dental pulp cells
DOI:
https://doi.org/10.1590/1678-7757-2024-0168Keywords:
Dental pulp, Matrix metalloproteinase-2, Mineral trioxide aggregate, PropolisAbstract
Objectives: This study sought to determine effects of Thai propolis extract mixed in mineral trioxide aggregate (MTA) on matrix metalloproteinase-2 (MMP-2) expression and its activity in inflamed human dental pulp cells (HDPCs). Materials and Methods: Interleukin-1β-primed HDPCs were treated with either the eluate of MTA mixed with distilled water, of MTA mixed with 0.75 mg/ml of the propolis extract, or of Dycal®, 0.75 mg/ml of the propolis extract, or 0.2% (v/v) of chlorhexidine for 24 or 72 h. The viability of HDPCs was determined by the PrestoBlue® cytotoxic assay. HDPCs’ lysates were analyzed for MMP-2 mRNA expression by RT-qPCR, while their supernatants were measured for MMP-2 activity by gelatin zymography. Results: At 24 and 72 h, a non-toxic dose of the propolis extract at 0.75 mg/ml by itself or mixed in MTA tended to reduce MMP-2 expression upregulated by MTA, while it further decreased the MMP-2 activity as compared to that of MTA mixed with distilled water. The MMP-2 activity of interleukin-1β-primed HDPCs treated with the eluate of the propolis extract mixed in MTA was significantly lower than that of interleukin-1β-primed HDPCs at 24 h (p=0.012). As a control, treatment with chlorhexidine significantly inhibited MMP-2 expression induced by MTA and MMP-2 activity enhanced by interleukin-1β (p<0.05). Treatment with Dycal® caused a significant increase in HDPC’s death, resulting in a significant decrease in MMP-2 expression and activity (p<0.05). Conclusions: MTA mixed with Thai propolis extract can reduce MMP-2 mRNA expression and activity when compared to MTA mixed with distilled water in inflamed HDPCs.
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References
- Palosaari H, Pennington CJ, Larmas M, Edwards DR, Tjäderhane L, Salo T. Expression profile of matrix metalloproteinases (MMPs) and tissue inhibitors of MMPs in mature human odontoblasts and pulp tissue. Eur J Oral Sci. 2003;111(2):117-27. doi: 10.1034/j.1600-0722.2003.00026.x
- Charadram N, Farahani RM, Harty D, Rathsam C, Swain MV, Hunter N. Regulation of reactionary dentin formation by odontoblasts in response to polymicrobial invasion of dentin matrix. Bone. 2012;50(1):265-75. doi: 10.1016/j.bone.2011.10.03
- Tjäderhane L, Buzalaf MA, Carrilho M, Chaussain C. Matrix metalloproteinases and other matrix proteinases in relation to cariology: the era of 'dentin degradomics'. Caries Res. 2015;49(3):193-208. doi: 10.1159/000363582
- Mazzoni A, Mannello F, Tay FR, Tonti GA, Papa S, Mazzotti G, et al. Zymographic analysis and characterization of MMP-2 and -9 forms in human sound dentin. J Dent Res. 2007;86(5):436-40. doi: 10.1177/154405910708600509
- Veerayutthwilai O, Byers MR, Pham TT, Darveau RP, Dale BA. Differential regulation of immune responses by odontoblasts. Oral Microbiol Immunol. 2007;22(1):5-13. doi: 10.1111/j.1399-302X.2007.00310.x
- Chang Y, Yang S, Hsieh Y. Regulation of matrix metalloproteinase-2 production by cytokines and pharmacological agents in human pulp cell cultures. J Endod. 2001;27(11):679-82. doi: 10.1097/00004770-200111000-00007
- Shin S, Lee J, Baek S, Lim S. Tissue levels of matrix metalloproteinases in pulps and periapical lesions. J Endod. 2002;28(4):313-5. doi: 10.1097/00004770-200204000-00013
- Huang FM, Yang SF, Hsieh YS, Liu CM, Yang LC, Chang YC. Examination of the signal transduction pathways involved in matrix metalloproteinases-2 in human pulp cells. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2004;97(3):398-403. doi: 10.1016/j.tripleo.2003.10.007
- Calin C, Sajin M, Moldovan VT, Coman C, Stratul SI, Didilescu AC. Immunohistochemical expression of non-collagenous extracellular matrix molecules involved in tertiary dentinogenesis following direct pulp capping: a systematic review. Ann Anat. 2021;23:151674 doi: 10.1016/j.aanat.2020.151674
- Cho SY, Seo DG, Lee SJ, Lee J, Lee SJ, Jung IY. Prognostic factors for clinical outcomes according to time after direct pulp capping. J Endod. 2013;39(3):327-31. doi: 10.1016/j.joen.2012.11.034
- Islam R, Islam MR, Tanaka T, Alam MK, Ahmed HM, Sano H. Direct pulp capping procedures– evidence and practice. Jpn Dent Sci Rev. 2023;(59):48-61. doi: 10.1016/j.jdsr.2023.02.002
- Okiji T, Yoshiba K. Reparative dentinogenesis induced by mineral trioxide aggregate: a review from the biological and physicochemical points of view. Int J Dent. 2009;464280:1-12. doi: 10.1155/2009/464280
- Andrei M, Vacaru RP, Coricovac A, Ilinca R, Didilescu AC, Demetrescu I. The effect of calcium-silicate cements on reparative dentinogenesis following direct pulp capping on animal models. Molecules. 2021;26(9):2725-53. doi: 10.3390/molecules26092725
- Parirokh M, Torabinejad M. Mineral trioxide aggregate: a comprehensive literature review--Part III: Clinical applications, drawbacks, and mechanism of action. J Endod. 2010;36(3):400-13. doi: 10.1016/j.joen.2009.09.009
- Accorinte ML, Loguercio AD, Reis A, Carneiro E, Grande RH, Murata SS, et al. Response of human dental pulp capped with MTA and calcium hydroxide powder. Oper Dent. 2008;33(5):488-95. doi: 10.2341/07-143
- Ramos AF, Miranda JL. Propolis: a review of its anti-inflammatory and healing actions. J Venom Anim Toxins incl Trop Dis. 2007;13(4):697-710. doi: 10.1590/S1678-91992007000400002
- Vagish Kumar L. Propolis in dentistry and oral cancer management. N Am J Med Sci. 2014;6(6):250-9. doi: 10.4103/1947-2714.134369
- Kantrong N, Kumtawee J, Damrongrungruang T, Puasiri S, Makeudom A, Krisanaprakornkit S, et al. An in vitro anti-inflammatory effect of Thai propolis in human dental pulp cells. J Appl Oral Sci. 2023;31:e20230006.1-12. doi: 10.1590/1678-7757-2023-0006
- Likitpongpipat N, Sangmaneedet S, Klanrit P, Noisombut R, Krisanaprakornkit S, Chailertvanitkul P. Promotion of dental pulp wound healing in New zealand white rabbits' teeth by Thai propolis product. J Vet Dent. 2019;36(1):17-24. doi: 10.1177/0898756418818891
- Kale Y, Yadav S, Dadpe M, Dahake P, Kendre S. Bioinductive and anti-inflammatory properties of propolis and Biodentine on SHED. Saudi Dent J. 2022;34(7):544-52. doi: 10.1016/j.sdentj.2022.08.009
- Shi B, Zhao Y, Yuan X. Effects of MTA and Brazilian propolis on the biological properties of dental pulp cells. Braz Oral Res. 2019;33:e117:1-9. doi: 10.1590/1807-3107bor-2019.vol33.0117
- Rahayu RP, Pribadi N, Widjiastuti I, Nugrahani NA. Combinations of propolis and Ca(OH) 2 in dental pulp capping treatment for the stimulation of reparative dentin formation in a rat model. F1000Res. 2020;9:1-12. doi: 10.12688/f1000research.22409.1
- Pribadi N, Budiarti D, Kurniawan HJ, Widjiastuti I. The NF-kB and collagen type 1 expression in dental pulp after treated calcium hydroxide combined with propolis. Eur J Dent. 2021;15(1):122-6. doi: 10.1055/s-0040-1716319
- International Organization for Standardization-ISO. ISO 10993-5:2009. Biological evaluation of medical devices. Part 5: Tests for in vitro cytotoxicity. Geneva: ISO; 2009. 33p
- International Organization for Standardization-ISO. ISO 10993-12:2012 Biological evaluation of medical devices. Part 12: Sample preparation and reference materials. Geneva: ISO; 2012. 21 p.
- Manaspon C, Jongwannasiri C, Chumprasert S, Sa-Ard-Iam N, Mahanonda R, Pavasant P, et al. Human dental pulp stem cell responses to different dental pulp capping materials. BMC Oral Health. 2021;21(1) 209. doi: 10.1186/s12903-021-01544-w
- Zhu L, Yang J, Zhang J, Peng B. A comparative study of BioAggregate and ProRoot MTA on adhesion, migration, and attachment of human dental pulp cells. J Endod. 2014;40(8):1118-23. doi: 10.1016/j.joen.2013.12.028
- Kim JM, Kang SW, Shin SM, Su Kim D, Choi KK, Kim EC, et al. Inhibition of matrix metalloproteinases expression in human dental pulp cells by all-trans retinoic acid. Int J Oral Sci. 2014;6(3):150-3. doi: 10.1038/ijos.2013.63
- Chang SW, Lee SY, Ann HJ, Kum KY, Kim EC. Effects of calcium silicate endodontic cements on biocompatibility and mineralization-inducing potentials in human dental pulp cells. J Endod. 2014;40(8):1194-200. doi: 10.1016/j.joen.2014.01.001
- Gusman H, Santana RB, Zehnder M. Matrix metalloproteinase levels and gelatinolytic activity in clinically healthy and inflamed human dental pulps. Eur J Oral Sci. 2002;110(5):353-7. doi: 10.1034/j.1600-0722.2002.21347.x
- Wisithphrom K, Windsor LJ. The effects of tumor necrosis factor-a, interleukin-1ß, interleukin-6, and transforming growth factor-ß1 on pulp fibroblast mediated collagen degradation. J Endod. 2006;32(9):853-61. doi: 10.1016/j.joen.2006.03.017
- Segueni N, Magid AA, Decarme M, Rhouati S, Lahouel M, Antonicelli F, et al. Inhibition of stromelysin-1 by caffeic acid derivatives from a propolis sample from Algeria. Planta Med. 2011;77(10):999-1004. doi: 10.1055/s-0030-1270713
- Saavedra N, Cuevas A, Cavalcante MF, Dörr FA, Saavedra K, Zambrano T, et al. Polyphenols from Chilean propolis and pinocembrin reduce MMP-9 gene expression and activity in activated macrophages. Biomed Res Int. 2016;2016:6505383. doi: 10.1155/2016/6505383
- Kim JM, Kang SW, Shin SM, Kim DS, Choi KK, Kim EC, et al. Inhibition of matrix metalloproteinases expression in human dental pulp cells by all-trans retinoic acid. Int J Oral Sci. 2014;6(3):150-3. doi: 10.1038/ijos.2013.63
- Vincenti MP. The matrix metalloproteinase (MMP) and tissue inhibitor of metalloproteinase (TIMP) genes. In: Clark IM, eds. Matrix metalloproteinase protocols. Methods in molecular biology ™. Totowa, NJ: Humana Press; 2001. v 151. p.121-48.
- Wu YI, Munshi HG, Sen R, Snipas SJ, Salvesen GS, Fridman R, et al. Glycosylation broadens the substrate profile of membrane type 1 matrix metalloproteinase. J Biol Chem. 2004;279(9):8278-89. doi: 10.1074/jbc.M311870200
- Gendron R, Grenier D, Sorsa T, Mayrand D. Inhibition of the activities of matrix metalloproteinases 2, 8, and 9 by chlorhexidine. Clin Diagn Lab Immunol. 1999;6(3):437-9. doi: 10.1128/CDLI.6.3.437-439.1999
- Retana-Lobo C, Guerreiro-Tanomaru JM, Tanomaru-Filho M, Mendes de Souza BD, Reyes-Carmona J. Sodium hypochlorite and chlorhexidine downregulate MMP expression on radicular dentin. Med Princ Pract. 2021;30(5):470-6. doi: 10.1159/000517887.
- Koo BH, Kim YH, Han JH, Kim DS. Dimerization of matrix functional implication in MMP-2 activation. J Biol Chem. 2012;287(27):22643-53. doi: 10.1074/jbc.M111.337949
- Mazzoni A, Mannello F, Tay FR, Tonti GA, Papa S, Mazzotti G, et al. Zymographic analysis and characterization of MMP-2 and -9 forms in human sound dentin. J Dent Res. 2007;86(5):436-40. doi: 10.1177/154405910708600509
- Zarra T, Lambrianidis T, Vasiliadis L, Gogos C. Effect of curing conditions on physical and chemical properties of MTA. Int Endod J. 2018;51(11):1279-91. doi: 10.1111/iej.12938
- Chen CL, Kao CT, Ding SJ, Shie MY, Huang TH. Expression of the inflammatory marker cyclooxygenase-2 in dental pulp cells cultured with mineral trioxide aggregate or calcium silicate cements. J Endod. 2010;36(3):465-8. doi: 10.1016/j.joen.2009
- Reyes-Carmona JF, Santos AR, Figueiredo CP, Felippe MS, Felippe WT, Cordeiro MM. In vivo host interactions with mineral trioxide aggregate and calcium hydroxide: inflammatory molecular signaling assessment. J Endod. 2011;37(9):1225-35. doi: 10.1016/j.joen.2011.05.031
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