The simultaneous miR-155-5p overexpression and miR-223-3p inhibition can activate pEMT in oral squamous cell carcinoma

Authors

  • Ruiman Zhou Xiamen Medical College, Department of Stomotology, Xiamen
  • Zhong Chen Xiamen Medical College, Department of Stomotology, Xiamen https://orcid.org/0000-0001-5896-7095
  • Yihuang Cai Xiamen Medical College, Department of Stomotology, Xiamen
  • Huilian Zhang Xiamen Medical College, Department of Stomotology, Xiamen
  • Shunjie Mao Xiamen Medical College, Department of Stomotology, Xiamen
  • Yunan Zhuang Xiamen Medical College, Department of Stomotology, Xiamen
  • Jiacheng Zheng Xiamen Medical College, Department of Stomotology, Xiamen

DOI:

https://doi.org/10.1590/

Keywords:

miR-223-3p, miR-155-5p, Migration, Oral squamous cell carcinoma, EMT

Abstract

Objective: This study aims to explore the effects of miR-223-3p and miR-155-5p on epithelial-mesenchymal transition (EMT) and migration in oral squamous cell carcinoma (OSCC). Methodology: EMT markers (E-cadherin, N-cadherin, P120 catenin (P120ctn), and vimentin) expression was determined by qRT-PCR and western blot analysis in SCC-9 cells which overexpress miR-155-5p and/or not express miR-223-3p. Scratch assays and Transwell migration assays were conducted to evaluate cell migration ability. Results: When miR-223-3p was inhibited in OSCC cells, P120ctn and E-cadherin mRNA levels were dramatically downregulated (P<0.05), while N-cadherin levels were significantly upregulated, and the migration ability of OSCC cells increased. The overexpression of miR-155-5p in OSCC cells upregulated miR-223-3p significantly (34-fold) compared to the control group. It also led to significant downregulation of the mRNA of P120ctn and E-cadherin and significant upregulation of the mRNA of N-cadherin and Vimentin (P<0.05). Meanwhile, the migratory ability of OSCC cells significantly increased. When miR-155-5p was overexpressed while miR-223-3p was inhibited, the highest expression of E-cadherin and P120ctn mRNA and the lowest expression of N-cadherin(P<0.05) was observed. Simultaneously, tumor cell migration was significantly facilitated. Conclusion: miR-223-3p inhibits the migration of OSCC cells, while miR-155-5p can elevate the miR-223-3p mRNA expression. The simultaneous miR-155-5p overexpression and miR-223-3p inhibition can activate pEMT, increasing OSCC migration in vitro. This provides a novel approach and potential target for the effective treatment of OSCC.

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Published

2024-10-21

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Vol. 32 (2024)

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Original Articles

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Copyright (c) 2024 Ruiman Zhou, Zhong Chen, Yihuang Cai, Huilian Zhang, Shunjie Mao, Yunan Zhuang, Jiacheng Zheng

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How to Cite

Zhou, R., Chen, Z., Cai, Y., Zhang, H., Mao, S., Zhuang, Y., & Zheng, J. (2024). The simultaneous miR-155-5p overexpression and miR-223-3p inhibition can activate pEMT in oral squamous cell carcinoma. Journal of Applied Oral Science, 32, e20240215. https://doi.org/10.1590/