Synergistic effect of Maquiberry cystatin, sodium fluoride and stannous chloride for the prevention of initial dental erosion in vitro

Vinícius Taioqui Pelá; Thelma Lopes Silva; Even Akemi Taira; Geórgia Almeida Sant’Ana; Letícia Oba Sakae; Flávia Mauad Levy; Taís Scaramucci Forlin; Eduardo Pereira de Souza; Talita Mendes Oliveira Ventura; Thiago Saads Carvalho; Adrian Lussi; Marília Afonso Rabelo Buzalaf

doi:10.1590/

Authors

Vinícius Taioqui Pelá Universidade de São Paulo. Faculdade de Odontologia de Bauru. Departamento de Ciências Biológicas https://orcid.org/0000-0001-7933-4422
Thelma Lopes Silva Universidade de São Paulo. Faculdade de Odontologia de Bauru. Departamento de Ciências Biológicas https://orcid.org/0009-0006-5622-2966
Even Akemi Taira Universidade de São Paulo. Faculdade de Odontologia de Bauru. Departamento de Ciências Biológica https://orcid.org/0000-0003-0853-1335
Geórgia Almeida Sant’Ana Universidade de São Paulo. Faculdade de Odontologia de Bauru. Departamento de Ciências Biológicas https://orcid.org/0009-0009-0688-9094
Letícia Oba Sakae University of Bern, School of Dental Medicine. Department of Restorative. Preventive and Pediatric Dentistry https://orcid.org/0000-0003-3885-4450
Flávia Mauad Levy Universidade de São Paulo, Faculdade de Odontologia de Bauru, Departamento de Ciências Biológicas https://orcid.org/0000-0002-4912-5272
Taís Scaramucci Forlin Universidade de São Paulo. Faculdade de Odontologia. Departamento de Odontologia Restaurador https://orcid.org/0000-0002-4445-6697
Eduardo Pereira de Souza Universidade Federal de São Carlos. Departamento de Genética e Evolução https://orcid.org/0000-0002-2013-0115
Talita Mendes Oliveira Ventura Universidade de São Paulo. Faculdade de Odontologia de Bauru. Departamento de Ciências Biológicas https://orcid.org/0000-0003-2101-1350
Thiago Saads Carvalho University of Bern, School of Dental Medicine. Department of Restorative, Preventive and Pediatric Dentistry https://orcid.org/0000-0002-2435-1169
Adrian Lussi University Hospital for Conservative Dentistry and Periodontology. Medical University of Innsbruck. https://orcid.org/0000-0002-0426-3364
Marília Afonso Rabelo Buzalaf Universidade de São Paulo. Faculdade de Odontologia de Bauru https://orcid.org/0000-0002-5985-3951

DOI:

https://doi.org/10.1590/

Keywords:

Demineralization, Enamel, Saliva

Abstract

Objective This study analyzed the synergistic effect between a recombinant Maquiberry (MaquiCPI-3) protein, sodium fluoride (NaF), and stannous chloride (SnCl2) against initial dental erosion in vitro. Methodology A total of 98 bovine enamel samples were prepared and allocated to seven treatment groups (n=14/group) as follows: deionized water (Water); commercial solution, 800 ppm Sn⁺², 500 ppm F-, Elmex^TM, Erosion, GABA, Therwil, BL, CH (Elmex); 500 ppm of fluoride (F^-) from NaF (NaF); 500 ppm of F^- from NaF and 800 ppm of tin (Sn⁺²) from SnCl₂ (NaF+SnCl); 0.5 mg/mL MaquiCPI-3 (MaquiCPI-3); combination of MaquiCPI-3 and NaF (Maqui+NaF) and; combination of MaquiCPI-3, NaF and SnCl (Maqui+NaF+SnCl). Samples were treated with the respective solutions (250 μl, 2 min, 37°C, 250 rpm). After forming the acquired enamel pellicle (AEP) by adding human saliva from nine volunteers (250 μl, 1 h, 37°C, 250 rpm), the samples underwent acid challenge (1 mL, 1% citric acid, pH 3.6, 1 min, 25°C, 250 rpm). All procedures were performed in triplicate. Surface microhardness change percentage (%SMC) and relative surface reflection intensity (%SRI) were measured and analyzed by One-way ANOVA/Tukey’s tests (p<0.05). Results The Elmex, NaF, NaF+SnCl, MaquiCPI-3, Maqui+NaF, and Maqui+NaF+SnCl groups showed significantly lower %SMC compared with Water. The NaF, Sn⁺², NaF+SnCl, MaquiCPI-3, Maqui+NaF and Maqui+NaF+SnCl groups exhibited significantly greater protection compared with Elmex. Maqui+NaF+SnCl revealed better enamel protection (significant) when compared with the MaquiCPI-3 and Maqui+NaF groups. Elmex, NaF+SnCl, MaquiCPI-3, Maqui+NaF, and Maqui+NaF+SnCl had a significantly higher %SRI compared to the Water and NaF groups, which did not differ between each other. Conclusion All treatment solutions provided protection against initial dental erosion in vitro. Formulations containing Maqui+NaF+SnCl₂ offered superior enamel protection compared with MaquiCPI-3 alone.

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Synergistic effect of Maquiberry cystatin, sodium fluoride and stannous chloride for the prevention of initial dental erosion in vitro

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