Evaluation of bleaching efficacy and cytotoxicity of an experimental hydrogel containing MnO-Doped Biosilicate® activated by violet LED light

Authors

  • Rafael Dascanio Universidade Estadual de Campinas (UNICAMP), Faculdade de Odontologia de Piracicaba
  • Rafael Antonio de Oliveira Ribeiro Universidade Estadual Paulista (UNESP), Faculdade de Odontologia de Araraquara, Departamento de Materiais Dentários e Prótese
  • Marina Trevelin Souza Universidade Federal de São Carlos (UFSCar), Departamento de Engenharia de Materiais, Laboratório de Materiais Vítreos (LaMaV)
  • Matheus Kury Universidade Paulista (UNIP), Departamento de Dentística
  • Edgar Dutra Zanotto Universidade Federal de São Carlos (UFSCar), Departamento de Engenharia de Materiais, Laboratório de Materiais Vítreos (LaMaV)
  • Carlos Alberto de Souza Costa Universidade Estadual Paulista (UNESP), Faculdade de Odontologia de Araraquara, Departamento de Fisiologia e Patologia
  • Vanessa Cavalli Universidade Estadual de Campinas (UNICAMP), Faculdade de Odontologia de Piracicaba, Departamento de Dentística

DOI:

https://doi.org/10.1590/

Keywords:

Hydrogen peroxide, Biosilicate, Cytotoxicity

Abstract

Although hydrogen peroxide (HP) at 35% is effective in dental bleaching, the cytotoxicity associated with its use remains a significant concern. In this context, this study aimed to develop a bleaching hydrogel containing 6% HP, Biosilicate® (BioS) doped with manganese oxide (MnO_BioS), and irradiated with violet LED light (LED). Enamel/dentin discs were submitted to the following bleaching treatments (n=08): 35%HP (positive control), 6%HP gel containing BioS or MnO_BioS (0 and 10 wt%), with or without LED irradiation. The discs were adapted to artificial pulp chambers (APCs), with the enamel exposed for bleaching and the dentin facing the culture medium (DMEM). Bleaching was performed in three 30-minute sessions with 7-day intervals. After bleaching, the extracts (DMEM + bleaching gel components diffused through the discs) were collected and applied to the odontoblast-like MDPC-23 cells. Color change (ΔE00) and changes in the whiteness index (ΔWID) were determined before (T0) and after the last bleaching session (T2). Cell viability (MTT, %), HP diffusion (µg/mL), oxidative cell stress (OxS), and cell fluorescence (live/dead assay, by confocal microscopy) were assessed after the first session (T1). The data were analyzed using ANOVA and Tukey’s test (p=0.05). The addition of 6%HP_MnO_BioS_LED exceeded ΔWID compared to 35%HP, showing statistical differences from the other groups, while ΔE00 was statistically similar to 35%HP. 6%HP_MnO_BioS_LED demonstrated higher cell viability, lower HP diffusion, and reduced oxidative stress (OxS) compared to the other groups (p<0.05). 6%HP_MnO_BioS_LED increased bleaching potential and presented lower cytotoxicity compared to 35%HP.

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Published

2026-03-02

Issue

Vol. 34 (2026)

Section

Original Articles

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How to Cite

Dascanio, R., Ribeiro, R. A. de O., Souza, M. T., Kury, M., Zanotto, E. D., Costa, C. A. de S., & Cavalli, V. (2026). Evaluation of bleaching efficacy and cytotoxicity of an experimental hydrogel containing MnO-Doped Biosilicate® activated by violet LED light. Journal of Applied Oral Science, 34, e20250503. https://doi.org/10.1590/