Association between polymorphisms in the gene encoding parathyroid hormone and orthodontically induced external apical root resorption

Authors

  • Veridiana Freitas Universidade da Região de Joinville (Univille). Departamento de Odontologia.
  • Luiz Fernando Fariniuk Universidade de Tuiuti do Paraná. Faculdade de Odontologia. Departamento de Ortodontia.
  • Michelle Nascimento Meger Universidade de Tuiuti do Paraná. Faculdade de Odontologia. Departamento de Ortodontia.
  • Angela Graciela Deliga Schroder Universidade de Tuiuti do Paraná. Faculdade de Odontologia. Departamento de Ortodontia.
  • Camila Guimarães da Costa Campos Universidade de Tuiuti do Paraná. Faculdade de Odontologia. Departamento de Ortodontia.
  • Rafaela Scariot Universidade Federal do Paraná. Faculdade de Odontologia.
  • Livia Azeredo A. Antunes Universidade da Região de Joinville (Univille). Departamento de Odontologia.
  • Bianca Marques de Araujo Universidade de Tuiuti do Paraná. Faculdade de Odontologia. Departamento de Ortodontia.
  • Christian Kirschneck University Hospital Bonn. Medical Faculty. Department of Orthodontics.
  • Erika Calvano Küchler University Hospital Bonn. Medical Faculty. Department of Orthodontics.
  • Flares Baratto-Filho Universidade da Região de Joinville (Univille). Departamento de Odontologia.

DOI:

https://doi.org/10.1590/1678-7765-2026-0424

Keywords:

Orthodontic treatment, Dental root, Gene, Polymorphism, Parathyroid hormone

Abstract

Introduction  The mechanism of action of parathyroid hormone (PTH) in bone resorption supports the idea that polymorphisms in this gene may be associated with root resorption. Therefore, this study aimed to investigate the association between polymorphisms in the gene encoding PTH and the occurrence of severe orthodontically induced external apical root resorption (OIEARR) in patients with severe skeletal malocclusion.Methodology  This cross-sectional study involved the evaluation of panoramic radiographs from orthodontic patients and DNA extracted from saliva. Post-treatment external apical root resorption was evaluated according to the Levander and Malmgren classification. Patients exhibiting Grade 4 resorption in at least one permanent tooth were included in the case group.Three polymorphisms in the PTH gene (rs694, rs6256, and rs307247) were investigated. DNA concentration and purity were quantified using a NanoDrop spectrophotometer, and genomic DNA was subsequently genotyped for allelic discrimination using TaqMan-based real-time PCR assays. Allele and genotype frequencies were calculated. Comparisons between groups with and without severe OIEARR were performed using the chi-square test and binary logistic regression analysis with a significance level of 5%. Results  A total of 159 patients (53 males and 106 females) were included. Most patients presented a Class III profile. Sex, age, and facial profile were not associated with severe OIEARR (p>0.05). A borderline association was observed in the allele distribution for the polymorphism rs6256 (p=0.057; OR=1.7, 95% CI=1.0–3.2). Binary logistic regression analysis did not show statistical significance (p>0.05). Conclusion  The G allele in the rs6256 polymorphism in the PTH gene showed borderline values for severe OIEARR. The multivariable model adjusted for sex and facial profile type did not show statistical associations between the studied PTH genetic polymorphisms and OIEARR.

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Published

2026-11-09

Issue

Vol. 34 (2026)

Section

Original Articles

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How to Cite

Freitas, V., Fariniuk, L. F., Meger, M. N., Deliga Schroder, A. G., Campos, C. G. da C., Scariot, R., Antunes, L. A. A., Araujo, B. M. de, Kirschneck, C., Küchler, E. C., & Baratto-Filho, F. (2026). Association between polymorphisms in the gene encoding parathyroid hormone and orthodontically induced external apical root resorption. Journal of Applied Oral Science, 34, e2026-0424. https://doi.org/10.1590/1678-7765-2026-0424