Evaluation of cytotoxicity of two endodontic cements in a macrophage culture

Authors

  • Celso Emanoel de Souza Queiroz University of Feira of Santana; Dental School
  • Janir Alves Soares Federal University of Valley of Jequitinhonha and Mucuri; Department of Dentstry
  • Renato de Toledo Leonardo UNESP; Dental School of Araraquara; Department of Endodontics
  • Iracilda Zeponi Carlos UNESP; School of Pharmaceutical Sciences of Araraquara; Discipline of Immunology
  • Welington Dinelli UNESP; Dental School of Araraquara; Department of Restorative Dentistry

DOI:

https://doi.org/10.1590/S1678-77572005000300007

Keywords:

Root canal filling materials, Nitric oxide, Macrophages

Abstract

Compared to gutta-percha, the endodontic cements are used in small quantity to seal root canals, but are indispensable to achieve hermetically sealed margins, where its biocompatibility depends on the sum of responses of each cell present in the periapical region. The object of this study was to evaluate the cytotoxicity of two endodontic cements, one based on epoxy resin (Sealer 26) and the other containing zinc oxide eugenol (Endofill) by using cultured peritoneal macrophages from Swiss mice to measure the induced production of nitric oxide. After solidification and pulverization, aliquots of 100mul of suspension containing 18mg/mL of the respective cements were added to 96-well tissue culture plates containing the tissue culture of macrophages at a concentration of 5.0X10(6) cells/ml. In the positive control group the cell culture was treated with 10mg/mL of lipopolyssaccharide from Escherichia coli 026:B6 and the cell culture alone represented the negative control. After 48 hours of incubation, at 37ºC, in 5% CO2, the cultures were placed in an ELISA automatic reader to evaluate the release of nitric oxide. The production of nitric oxide for cement Sealer 26 was between 36.1 and 313.0 mumols, with a mean of 143.82±111.03mumols, while for the Endofill these values were significantly less (p=0.01), varying from 50.8 to 125.7mumols, with a mean of 80.33±28.42 mumols. In the positive and negative control groups the mean release of nitric oxide was of 162.75mumols and 4.42mumols, respectively. There was no significant difference between the positive control group and cement Sealer 26 (p>;0.05). Therefore, the cement Sealer 26 caused significantly greater toxicity to the macrophages, possibly due to the components from the epoxy resin and formaldehyde release during polymerization.

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Published

2005-09-01

Issue

Section

Original Articles

How to Cite

Evaluation of cytotoxicity of two endodontic cements in a macrophage culture . (2005). Journal of Applied Oral Science, 13(3), 237-242. https://doi.org/10.1590/S1678-77572005000300007