A model system to study antimicrobial strategies in endodontic biofilms

Authors

  • Carlos Estrela Federal University of Goiás; Department of Oral Science
  • Gilson Blitzkow Sydney Federal University of Paraná; Department of Endodontics
  • José Antonio Poli Figueiredo Pontifical Catholic University of Rio Grande do Sul
  • Cyntia Rodrigues de Araújo Estrela Federal University of Goiás; Biological Science Institute

DOI:

https://doi.org/10.1590/S1678-77572009000200003

Keywords:

Biofilm, Endodontic biofilm, Enterococcus faecalis, Intracanal medicaments, Antimicrobial strategies

Abstract

The purpose of this work was to develop a model system to study antimicrobial strategies in endodontic biofilms. Enterococcus faecalis suspension was colonized in 10 human root canals. Five milliliters of Brain Heart Infusion (BHI) were mixed with 5 mL of the bacterial inoculums (E. faecalis) and inoculated with sufficient volume to fill the root canal during 60 days. This procedure was repeated every 72 h, always using 24-h pure culture prepared and adjusted to No. 1 MacFarland turbidity standard. Biofilm formation was analyzed by scanning electron microscopy (SEM). E. faecalis consistently adhered to collagen structure, colonized dentin surface, progressed towards the dentinal tubules and formed a biofilm. The proposed biofilm model seems to be viable for studies on antimicrobial strategies, and allows for a satisfactory colonization time of selected bacterial species with virulence and adherence properties.

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Published

2009-04-01

Issue

Vol. 17 No. 2 (2009)

Section

Original Articles

License

Todo o conteúdo do periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons do tipo atribuição CC-BY.

 

 

How to Cite

A model system to study antimicrobial strategies in endodontic biofilms . (2009). Journal of Applied Oral Science, 17(2), 87-91. https://doi.org/10.1590/S1678-77572009000200003