Immunohistochemical analysis for CD21, CD35, Caldesmon and S100 protein on dendritic cells types in oral lymphomas

Authors

  • Ricardo Alves Mesquita Federal University of Minas Gerais; Dental School; Department of Oral Surgery, Oral Medicine and Oral Pathology
  • Vera Cavalcanti de Araújo School and Research Center of Dentistry São Leopoldo Mandic; Department of Oral Pathology
  • Roberto Antônio Pinto Paes Hospital of Santa Casa of São Paulo; Department of Pathological Anatomy
  • Fábio Daumas Nunes University of Sao Paulo; Dental School; Department of Oral Pathology
  • Suzana Cantanhede Orsini Machado de Souza University of Sao Paulo; Dental School; Department of Oral Pathology

DOI:

https://doi.org/10.1590/S1678-77572009000300023

Keywords:

CD21, CD35, Follicular dendritic cells, Interdigitating dendritic cells, Immunohistochemistry, Lymphoma, Oral lymphomas

Abstract

OBJECTIVE: Follicular dendritic cells (FDCs) and interdigitating dendritic cells (IDCs) are dendritic cells found in lymphoid follicles, reactive follicles and in lymphomas. The goal of this study was to evaluate the presence and distribution of FDCs and IDCs in oral lymphomas. MATERIAL AND METHODS: Immunohistochemistry reactions were applied to 50 oral lymphomas using the antibodies anti-CD21, anti-CD35 and anti-caldesmon to FDCs, and anti-S100 protein to IDCs. Caldesmon+/FDCs and S100+/IDCs were quantified in Imagelab® software. RESULTS: FDCs revealed by CD21 and CD35 were positively stained in two cases of diffuse large B-cell lymphoma, one MALT lymphoma, and in one case of mantle cell lymphoma. FDCs were immunopositive to caldesmon in all cases, as well as IDCs to S100 protein. Burkitt lymphoma presented a lower amount of caldesmon+/FDCs and S100+/IDCs than diffuse large B-cell lymphoma and plasmablastic lymphoma of the oral mucosa type. CONCLUSIONS: The microenvironment determined by neoplastic lymphoid cells in oral lymphomas is responsible by the development and expression of dendritic cells types.

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Published

2009-06-01

Issue

Vol. 17 No. 3 (2009)

Section

Original Articles

License

Todo o conteúdo do periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons do tipo atribuição CC-BY.

 

 

How to Cite

Immunohistochemical analysis for CD21, CD35, Caldesmon and S100 protein on dendritic cells types in oral lymphomas . (2009). Journal of Applied Oral Science, 17(3), 248-253. https://doi.org/10.1590/S1678-77572009000300023