Time- and concentration-dependent cytotoxicity of antibiotics used in endodontic therapy

Authors

  • Marina Beloti Ferreira University of São Paulo; Dental School; Department of Restorative Dentistry
  • Suely Myiagi University of São Paulo; Dental School; Department of Restorative Dentistry
  • Carlos Goes Nogales University of São Paulo; Dental School; Department of Restorative Dentistry
  • Marcia Sampaio Campos University of São Paulo; Dental School; Department of Restorative Dentistry
  • José Luiz Lage-Marques University of São Paulo; Dental School; Department of Pathology

DOI:

https://doi.org/10.1590/S1678-77572010000300011

Keywords:

Cytotoxicity, Ciprofoxacin, Clyndamicin, Metronidazole

Abstract

OBJECTIVE: New drugs have to be assessed in endodontic therapy due to the presence of microorganisms resistant to therapeutic procedures. Thus, this study evaluated the time- and concentration-dependent cytotoxicity of different antibiotics used in endodontic therapy. MATERIAL AND METHODS: Human gingival fibroblasts were treated and divided into the following experimental groups: Group I - control; Group II - ciprofoxacin hydrochloride; Group III - clyndamicin hydrochloride; and Group IV - metronidazole. Each drug was used at concentrations of 5, 50, 150, and 300 mg/L for 24, 48, 72, and 96 h. Cytotoxicity was evaluated by the MTT assay [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] and spectrophotometric reading of ELISA plates. The results were analyzed by BioEstat 4.0 software using Kruskal-Wallis and Dunn's tests at a signifcance level of 5%. Cell viability was assessed for the different concentrations and times. RESULTS: All drugs presented dose-dependent cytotoxicity. Concentrations of 5 and 50 mgjL produced viable fibroblasts at all experimental times in all groups. CONCLUSIONS: Cell viability at 24 h was greater than in the other experimental times. Comparison between the same concentrations of antibiotics at different times showed that metronidazole presented the highest cell viability at 72 and 96 h compared to the other antibiotics, whereas clyndamicin hydrochloride showed higher cell viability at 72 h than ciprofoxacin hydrochloride.

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Published

2010-06-01

Issue

Section

Original Articles

How to Cite

Ferreira, M. B., Myiagi, S., Nogales, C. G., Campos, M. S., & Lage-Marques, J. L. (2010). Time- and concentration-dependent cytotoxicity of antibiotics used in endodontic therapy . Journal of Applied Oral Science, 18(3), 259-263. https://doi.org/10.1590/S1678-77572010000300011