Radiological and histopathological evaluation of experimentally-induced periapical lesion in rats

Autores

  • Renata Cordeiro Teixeira University of São Paulo; Bauru School of Dentistry; Department of Stomatology
  • Cassia Maria Fischer Rubira University of São Paulo; Bauru School of Dentistry; Department of Stomatology
  • Gerson Francisco Assis University of São Paulo; Bauru School of Dentistry; Department of Biological Sciences
  • José Roberto Pereira Lauris University of São Paulo; Bauru School of Dentistry; Department of of Pediatric Dentistry, Orthodontics and Community Health
  • Tania Mary Cestari University of São Paulo; Bauru School of Dentistry; Department of Biological Sciences
  • Izabel Regina Fischer Rubira-Bullen University of São Paulo; Bauru School of Dentistry; Department of Stomatology

DOI:

https://doi.org/10.1590/S1678-77572011005000020

Palavras-chave:

Radiology, Periapical diseases, Histology, Rats

Resumo

OBJECTIVE: This study evaluated experimentally-induced periapical bone loss sites using digital radiographic and histopathologic parameters. MATERIAL AND METHODS: Twenty-seven Wistar rats were submitted to coronal opening of their mandibular right first molars. They were radiographed at 2, 15 and 30 days after the operative procedure by two digital radiographic storage phosphor plates (Digora®). The images were analyzed by creating a region of interest at the periapical region of each tooth (ImageJ) and registering the corresponding pixel values. After the sacrifice, the specimens were submitted to microscopic analysis in order to confirm the pulpal and periapical status of the tooth. RESULTS: There was significant statistically difference between the control and test sides in all the experimental periods regarding the pixel values (two-way ANOVA; p<0.05). CONCLUSIONS: The microscopic analysis proved that a periapical disease development occurred during the experimental periods with an evolution from pulpal necrosis to periapical bone resorption.

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Publicado

2011-10-01

Edição

v. 19 n. 5 (2011)

Seção

Artigos Originais

Licença

Todo o conteúdo do periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons do tipo atribuição CC-BY.

 

 

Como Citar

Radiological and histopathological evaluation of experimentally-induced periapical lesion in rats . (2011). Journal of Applied Oral Science, 19(5), 500-504. https://doi.org/10.1590/S1678-77572011005000020