Human tooth germ stem cell response to calcium-silicate based endodontic cements

Authors

  • Esra Pamukcu Guven Yeditepe University; Faculty of Dentistry; Department of Endodontics
  • Mehmet Emir Yalvac Nationwide Children's Hospital; Research Institute; Center for Gene Therapy
  • Mehmet Baybora Kayahan Yeditepe University, Istanbul; Faculty of Dentistry; Department of Endodontics
  • Hakk Sunay Yeditepe University, Istanbul; Faculty of Dentistry; Department of Endodontics
  • Fikrettin SahIn Yeditepe University; College of Engineering and Architecture; Department of Genetics and Bioengineering
  • Gunduz Bayirli Yeditepe University, Istanbul; Faculty of Dentistry; Department of Endodontics

DOI:

https://doi.org/10.1590/1678-775720130047

Abstract

OBJECTIVE: The aim of this study was to compare the cytotoxic effects of endodontic cements on human tooth germ stem cells (hTGSCs). MTA Fillapex, a mineral trioxide aggregate (MTA)-based, salicylate resin containing root canal sealer, was compared with iRoot SP, a bioceramic sealer, and AH Plus Jet, an epoxy resin-based root canal sealer. MATERIAL AND METHODS: To evaluate cytotoxicity, all materials were packed into Teflon rings (4 mmµ3 mm) and co-cultured with hTGSCs with the aid of 24-well Transwell permeable supports, which had a pore size of 0.4 µm. Coverslips were coated with MTA Fillapex, iRoot SP and AH Plus Jet and each coverslip was placed onto the bottom of one well of a six-well plate for scanning electron microscopy (SEM) analysis. Before the cytotoxicity and SEM analysis, all samples were stored at 37ºC and at 95% humidity and 5% CO2 for 24 hours to set. The cellular viability was analyzed using MTS test (3-(4,5-dimethyl-thiazol-2-yl)-5-(3-carboxy-methoxy-phenyl)-2-(4-sulfo-phenyl)-2H-tetrazolium). The cytotoxic effects and SEM visualization of the tested materials were analyzed at 24-hour, 72-hour, one-week and two-week periods. RESULTS: On the 1st day, only MTA Fillapex caused cytotoxicity compared to negative control (NC) group (p<0.008). No significant difference was observed between the other tested materials at this period (p>;0.05). After 14 days of incubation with the test materials, MTA Fillapex exhibited significantly higher cytotoxicity compared with iRoot SP, AH Plus Jet and the NC group (P<0.008). In the SEM analysis, the highest levels of cell attachment were observed for iRoot SP and the control group. After 24 hours, MTA Fillapex reduced the number of cells attached to the surface. CONCLUSIONS: Within the limitations of this study, sealers exerted different cytotoxic effects on hTGSCs. Although all materials have exerted cellular toxicity, iRoot SP and AH Plus Jet may promote better attachment to hTGSCs.

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Published

2013-07-01

Issue

Section

Original Articles

How to Cite

Guven, E. P., Yalvac, M. E., Kayahan, M. B., Sunay, H., SahIn, F., & Bayirli, G. (2013). Human tooth germ stem cell response to calcium-silicate based endodontic cements . Journal of Applied Oral Science, 21(4), 351-357. https://doi.org/10.1590/1678-775720130047