Influence of temperature and culture media on growth and lipolytic activity of deep-sea Halomonas sulfidaeris LAMA 838 and Marinobacter excellens LAMA 842
DOI:
https://doi.org/10.1590/S2675-28242020068282Keywords:
Deep-sea bacteria, microbial growth, lipolytic activity, PsychrotolerantsAbstract
Bacteria with lipolytic activity are widespread in the marine environment. These organisms can be used
as a source of lipases with activity in unusual conditions such as low temperatures and high salinities.
These characteristics allow them to be applied to several industrial processes and products including
wastewater treatment and detergent production. In this context the lipolytic activity of bacteria isolated
from deep-sea sediments of the South Atlantic was evaluated. Among the collection studied, two strains,
Halomonas sulfidaeris LAMA 838 and Marinobacter excellens LAMA 842, were selected for further analysis
concerning their growth and lipolytic activity in different temperatures and culture media. Both strains
were characterized as mesophiles: H. sulfidaeris LAMA 838 grew best at 30ºC, while M. excellens LAMA 842
showed a maximum growth rate between 20 and 25ºC. Maximum lipolytic activity for both microorganisms
was observed above 45ºC, but only M. excellens LAMA 842 had lipolytic activity at low temperatures (5ºC) as
well. Considering the culture media employed, H. sulfidaeris LAMA 838 grew better in marine broth without
Tween 40, while M. excellens LAMA 842 grew best in marine broth supplemented with Tween 40. Both
organisms had maximum lipolytic activity in rich or minimal media supplemented with Tween 40. These
results demonstrate the potential of deep-sea bacteria as sources of lipases and indicate conditions that
may be used in their cultivation and for the production of industrially relevant enzymes.
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