Method for immobilizing invertase by adsorption on Dowex® anionic exchange resin

Authors

  • Ester Junko Tomotani University of São Paulo; School of Pharmaceutical Sciences; Department of Biochemical and Pharmaceutical Technology
  • Michele Vitolo University of São Paulo; School of Pharmaceutical Sciences; Department of Biochemical and Pharmaceutical Technology

DOI:

https://doi.org/10.1590/S1516-93322006000200009

Keywords:

Invertase, Immobilization, Anionic exchange resins, Adsorption

Abstract

This communication describes a method for adsorbing the invertase (EC.3.2.1.26) on DOWEX® anion exchange resin. Among the types of DOWEX® resins studied (1x8:50-400; 1x4:50-400 and 1x2:100-400), 1X4-200 was the most suitable, because it adsorbed the invertase molecules completely and the complex 1X4-200/invertase retained 100% of the catalytic activity. Moreover, no leakage of enzyme from the support was noted at the end of the sucrose hydrolysis.

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Published

2006-06-01

Issue

Vol. 42 No. 2 (2006)

Section

Original Papers

How to Cite

Method for immobilizing invertase by adsorption on Dowex® anionic exchange resin. (2006). Revista Brasileira De Ciências Farmacêuticas, 42(2), 245-249. https://doi.org/10.1590/S1516-93322006000200009