Nephrotoxicity Of Polymyxin B: Experimental Study In Cells And Implications For Nursing Practice
DOI:
https://doi.org/10.1590/S0080-6234201400002000011Abstract
The aim of the study was to characterize the cell damage mechanisms involved in the pathophysiology of cytotoxicity of polymyxin B in proximal tubular cells (LLC - PK1) and discuss about the nurses interventions to identify at risk patients and consider prevention or treatment of nephrotoxicity acute kidney injury. This is a quantitative experimental in vitro study, in which the cells were exposed to 375μM polymyxin B sulfate concentration. Cell viability was determined by exclusion of fluorescent dyes and morphological method with visualization of apoptotic bodies for fluorescence microscopy. Cells exposed to polymyxin B showed reduced viability, increased number of apoptotic cells and a higher concentration of the enzyme lactate dehydrogenase. The administration of polymyxin B in vitro showed the need for actions to minimize adverse effects such as nephrotoxicity.Downloads
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Published
2014-04-01
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How to Cite
Neiva, L. B. de M., Borges, F. T., Watanabe, M., Pessoa, E. de A., Barbosa, D. A., & Vattimo, M. de F. F. (2014). Nephrotoxicity Of Polymyxin B: Experimental Study In Cells And Implications For Nursing Practice . Revista Da Escola De Enfermagem Da USP, 48(2), 272-277. https://doi.org/10.1590/S0080-6234201400002000011