Replantation model after warm ischemia in rats and the effectiveness of allopurinol, streptoquinase and hyperbaric oxigen therapy
DOI:
https://doi.org/10.11606/issn.1679-9836.v80i2-4p78-88Keywords:
Rats, Wistar, Replantation/methods, Extremities/surgery, Leg/transplantation, Ischemia/surgery, Allopurinol/therapeutic use, Hyperbaric Oxigenation/methods, Streptokinase/therapeutic use.Abstract
Prolonged ischemia leads to alterations in the tecidual microcirculation and oxigen free radicals production. It was tested a new replantation model after warm ischemia in rats and the effectiveness of allopurinol, streptoquinase and hyperbaric oxigen therapy. Amputation with preservation of vessels and nerves of the right hind limb was proceeded
in 110 rats. The groups MG1, MG2, MG3, MG4 and MG5 were submitted to 0, 2, 4, 6 and 8 hours of ischemia. The limbs
were observed for 7 days and the survival limb rates in each group were 100%, 80%, 63,6%, 50% and 20% respectively.
MG4 was elected control for experimental groups. The groups EG1, EG2 and EG3 were submitted to 6 hours of ischemia and were treated with streptoquinase , allopurinol and hyperbaric oxigen therapy. The survival limb rates were 66,7%, 70% and 30% respectively. The results suggest that the administration of streptoquinase and allopurinol may be helpful to improve limb salvage after ischemia while hyperbaric oxigen therapy may not.
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