Radiometric markers of DNA damage: possible targets and current status
DOI:
https://doi.org/10.11606/issn.1679-9836.v94i1p46-56Keywords:
DNA damage, Radiation, ionizing, DNA repair.Abstract
Either by endogenous or exogenous, therapeutic or spontaneous sources, the damage to cellular DNA occurs every time, requires repair with specific enzymes and eventually produces neoplastic processes. In cancer treatment, the benefits of radiotherapy and radiomimetic therapy must be compared with the risks of mutations de novo. It is estimated that individuals submitted to radiotherapy present 5% risk of developing secondary tumors. In the present review, 22 articles were selected from bibliographic database PubMed, SciELO, and LILACS using three subject descriptors: DNA damage, ionizing radiation and DNA repair, between 2002 and 2014. Results: based on cascades of repair of DNA damage, we analyzed the kinetic properties of repair proteins detected in vitro ATM, BRCA1, p53, histone H2AX, MDC1 and 53BP1. The ATM and MDC1 proteins evidenced a brief plateau, and ATM demonstrated two periods of concentration changes. Conclusion: p53, 53BP1, BRCA1 and H2AX represent a potential novel for indirect detection of damage after radiotherapy, as they showed a longstanding plateau.
