Evaluation of the chemical composition of a copaiba oil (Copaifera spp.) and its effect on mesenchymal stem cells

Authors

DOI:

https://doi.org/10.11606/issn.1679-9836.v101i5e-185868

Keywords:

Ethnopharmacology, In vitro techniques, Plants, Sesquiterpenes, Stem cells, Stromal cells, Traditional medicine, Toxicity

Abstract

Oleoresin obtained from Copaifera trees is extensively used in Brazilian traditional medicine. This study has evaluated the chemical composition and effect of Copaifera officinalis oleoresin on stem cells. The oleoresin was analyzed by Gas Chromatography (GC) and the cells were treated with the oleoresin at concentrations of 0.5, 20, 110, 140, 170 or 200 µg/ml for 24h for cellular tests. GC identified the sesquiterpenes beta-caryophyllene, trans-alpha-bergamotene, and caryophyllene oxide II as the main compounds in oleoresin. The cell viability and cytotoxicity assays showed the lowest concentrations of oleoresin used presented similar results to the control group and the higher concentrations tested significantly decreased cell viability and increased cytotoxicity. In a conclusion, the main components found in copaiba oleoresin were sesquiterpenes and the low tested concentrations were not cytotoxic. The increased concentrations of copaiba oleoresin promoted a decrease in cell viability and an increase of cytotoxicity in the stem cells. Although copaiba oleoresin has ethnopharmacology use in healing, this study showed toxicity in stem cells, which are related to the corporeal regeneration process. Therefore, caution must be taken with the dosage of the oleoresin to be used since this in vitro study showed cytotoxicity and a negative impact on stem cell viability at the higher tested concentrations.

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Author Biographies

  • Natasha Maurmann, Federal University of Rio Grande do Sul

    Laboratório de Hematologia e Células-tronco, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brasil. 
    Programa de Pós-Graduação em Fisiologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brasil.

  • Daniela Guerra Lund, Universidade Federal do Rio Grande do Sul. Faculdade de Farmácia, Laboratório de Hematologia e Células-Tronco

    Laboratório de Hematologia e Células-Tronco, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brasil.

  • Daniela Pavulack Pereira, Universidade Federal do Rio Grande do Sul. Faculdade de Farmácia, Laboratório de Hematologia e Células-Tronco

    Laboratório de Hematologia e Células-Tronco, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brasil. 
    Programa de Pós-Graduação em Patologia, Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Porto Alegre, RS, Brasil.

  • Patricia Pranke, Universidade Federal do Rio Grande do Sul. Faculdade de Farmácia, Laboratório de Hematologia e Células-Tronco

    Laboratório de Hematologia e Células-Tronco, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brasil. 
    Programa de Pós-Graduação em Fisiologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brasil.
    Instituto de Pesquisa com Células-tronco (IPCT), Porto Alegre, RS, Brasil.

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Published

2022-09-02

Issue

Vol. 101 No. 5 (2022)

Section

Artigos Originais/Originals Articles

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Copyright (c) 2022 Natasha Maurmann, Daniela Guerra Lund, Daniela Pavulack Pereira, Patricia Pranke

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This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

How to Cite

Maurmann, N., Lund, D. G., Pereira, D. P., & Pranke, P. . (2022). Evaluation of the chemical composition of a copaiba oil (Copaifera spp.) and its effect on mesenchymal stem cells. Revista De Medicina, 101(5), e-185868. https://doi.org/10.11606/issn.1679-9836.v101i5e-185868