Shotgun metagenomic sequencing of the first case of monkeypox virus in Brazil, 2022
DOI:
https://doi.org/10.1590/S1678-9946202264048%20Keywords:
Viral metagenomics, Monkeypox virus, West African clade, Genomic surveillance, BrazilAbstract
Monkeypox virus (MPXV), a zoonotic virus endemic to the African continent, has been reported in 33 non-endemic countries since May 2022. We report an almost complete genome of the first confirmed case of MPXV in Brazil. Shotgun metagenomic sequencing was completed in 18 hours, from DNA extraction to consensus sequence generation.
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Copyright (c) 2022 Ingra Morales Claro, Camila Malta Romano, Darlan da Silva Candido, Evelyn Lepka de Lima, José Angelo Lauletta Lindoso, Mariana Severo Ramundo, Filipe Romero Rebello Moreira, Luiz Alberto Costa Barra, Luciana Marques Sansão Borges, Lucas Alberto Medeiros, Marcia Y. S. Tomishige, Tomas Moutinho, Anderson José Dias da Silva, Camila Cristina Martini Rodrigues, Luiz Cesar Fernandes de Azevedo, Lucy Santos Villas-Boas, Camila Alves Maia da Silva, Thaís Moura Coletti, Erika R. Manuli, Aine O’Toole, Joshua Quick, Nicholas Loman, Andrew Rambaut, Nuno R. Faria, Claudia Figueiredo-Mello, Ester Cerdeira Sabino
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Funding data
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Fundação de Amparo à Pesquisa do Estado de São Paulo
Grant numbers 18/14389018/143890 -
Bill and Melinda Gates Foundation
Grant numbers INV-034540 and INV-034652 -
Conselho Nacional de Desenvolvimento Científico e Tecnológico
Grant numbers 404096/2020-4 -
Conselho Nacional de Desenvolvimento Científico e Tecnológico
Grant numbers 402794/2020-6