Detection of Plasmodium in Anopheles spp (Diptera: Nematocera: Culicidae) using molecular biology techniques: a systematic review

Authors

  • Erique da Costa Fonseca Universidade Federal do Amapá, Programa de Pós-Graduação em Biodiversidade e Biotecnologia da Amazônia Legal, Macapá, Amapá, Brazil; Universidade Federal do Amapá, Laboratório de Arthropoda, Macapá, Amapá, Brazil https://orcid.org/0000-0001-8906-5246
  • Everson dos Santos David Universidade Federal do Amapá, Programa de Pós-Graduação em Biodiversidade e Biotecnologia da Amazônia Legal, Macapá, Amapá, Brazil; Universidade Federal do Amapá, Laboratório de Arthropoda, Macapá, Amapá, Brazil https://orcid.org/0000-0002-9636-9974
  • Karen Carmo dos Santos Universidade Federal do Amapá, Laboratório de Arthropoda, Macapá, Amapá, Brazil; Universidade Federal do Amapá, Programa de Pós-Graduação em Ciências Farmacêuticas, Macapá, Amapá, Brasil https://orcid.org/0009-0000-8542-765X
  • Franciane Pereira de Oliveira Universidade Federal de São Paulo, Laboratório de Retrovirologia, São Paulo, São Paulo, Brazil https://orcid.org/0000-0002-5414-3280
  • Ledayane Mayana Costa Barbosa Universidade Federal do Amapá, Laboratório de Arthropoda, Macapá, Amapá, Brazil; Universidade Federal do Amapá, Programa de Pós-Graduação em Biociências, Macapá, Amapá, Brazil https://orcid.org/0000-0003-2430-8774
  • Shirley Vasconcelos Komninakis Universidade Federal do Amapá, Programa de Pós-Graduação em Biodiversidade e Biotecnologia da Amazônia Legal, Macapá, Amapá, Brazil; Universidade Federal de São Paulo, Laboratório de Retrovirologia, São Paulo, São Paulo, Brazil https://orcid.org/0000-0001-7784-3418
  • Raimundo Nonato Picanço Souto Universidade Federal do Amapá, Programa de Pós-Graduação em Biodiversidade e Biotecnologia da Amazônia Legal, Macapá, Amapá, Brazil; Universidade Federal do Amapá, Laboratório de Arthropoda, Macapá, Amapá, Brazil https://orcid.org/0000-0002-8795-1217

DOI:

https://doi.org/10.1590/S1678-9946202567071

Keywords:

Molecular biology, Plasmodium, Malaria, Anopheles

Abstract

This study aims to find evidence of the effectiveness of molecular biology techniques in detecting Plasmodium in Anopheles spp mosquitoes. This systematic review was based on the PRISMA 2020 protocol. It was carried out in five electronic databases (LILACS, PubMed, SciELO, Scopus, and Web of Science, with published studies in health and interdisciplinary areas) in addition to complementary research on Google Scholar. Studies that used molecular biology techniques to detect and evaluate Plasmodium in anopheles (the results of which determined the type of Plasmodium in the samples) were included in this review. In total, 484 recent studies were retrieved from the electronic databases. According to the inclusion/exclusion criteria, only 12 studies met the objectives of this systematic review. Molecular biology was used in mosquitoes to determine parasitic species in all studies. Despite the difficulties and challenges in using molecular biology in mosquitoes, the obtained scientific advances show the accuracy and reliability of the results, contributing to an effective epidemiological response and monitoring of the spread of Plasmodium in endemic areas.

Downloads

Download data is not yet available.

Published

2025-11-07

Issue

Vol. 67 (2025)

Section

Original Article

License

Copyright (c) 2025 Erique da Costa Fonseca, Everson dos Santos David, Karen Carmo dos Santos, Franciane Pereira de Oliveira, Ledayane Mayana Costa Barbosa, Shirley Vasconcelos Komninakis, Raimundo Nonato Picanço Souto

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

How to Cite

Fonseca, E. da C., David, E. dos S., Santos, K. C. dos, Oliveira, F. P. de, Barbosa, L. M. C., Komninakis, S. V., & Souto, R. N. P. (2025). Detection of Plasmodium in Anopheles spp (Diptera: Nematocera: Culicidae) using molecular biology techniques: a systematic review. Revista Do Instituto De Medicina Tropical De São Paulo, 67, e71. https://doi.org/10.1590/S1678-9946202567071