Comparative studies of Yersinia pestis outer membrane isolation techniques and their potential use in plague epidemiology

Authors

  • Frederico Guilherme Coutinho Abath Fundação Oswaldo Cruz; Centro de Pesquisas Aggeu Magalhães
  • Luís Carlos de Sousa Ferreira Universidade Federal de Pernambuco; Departamento de Biofísica e Radiobiologia; Laboratório de Imunopatologia Keizo Asami

Keywords:

Yersinia pestis, outer membrane proteins, membrane isolation techniques

Abstract

In the present study three techniques for obtaining outer membrane enriched fractions from Yersinia pestis were evaluated. The techniques analysed were: differential solubilization of the cytoplasmic membrane with Sarkosyl or Triton X-100, and centrifugation in sucrose density gradients. The sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of outer membrane isolated by the different methods resulted in similar protein patterns. The measurement of NADH-dehydrogenase and succinate dehydrogenase (inner membrane enzymes) indicated that the outer membrane preparations obtained by the three methods were pure enough for analytical studies. In addition, preliminary evidences on the potential use of outer membrane proteins for the identification of geographic variants of Y. pestis wild isolates are presented.

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Published

1990-04-01

Issue

Section

Original Article

How to Cite

Abath, F. G. C., & Ferreira, L. C. de S. (1990). Comparative studies of Yersinia pestis outer membrane isolation techniques and their potential use in plague epidemiology . Revista Do Instituto De Medicina Tropical De São Paulo, 32(2), 78-83. https://revistas.usp.br/rimtsp/article/view/28714