Staining of intestinal protozoa with heidenhain's iron Hematoxylin
Keywords:
Iron hematoxylin staining, Intestinal protozoaAbstract
Due to its unique properties, iron hematoxylin has been traditionally recommended for staining intestinal protozoa. This process can be simplified by reducing the number of steps and periods of permanence of the slides in some of the liquids used, without detriment to the quality of the results. Thus iron hematoxylin becomes adequate for routine use. Hematoxylin is a natural dye extracted from Haematoxylon campechianum, of the family Leguminosae. It must first be 'ripened', i.e. oxidized to hematein, which reacts with ferric ammonium sulphate to produce the ferric lake (iron hematoxylin), a basic dye. Iron hematoxylin most frequently stains regressively, i.e. the slides are first overstained and then differentiated.Downloads
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Published
2003-01-01
Issue
Section
Technical Report
How to Cite
Ferreira, C. S. (2003). Staining of intestinal protozoa with heidenhain’s iron Hematoxylin . Revista Do Instituto De Medicina Tropical De São Paulo, 45(1), 43-44. https://revistas.usp.br/rimtsp/article/view/30669