Mayaro virus: imported cases of human infection in São Paulo State, Brazil

Authors

  • Terezinha Lisieux M. Coimbra Instituto Adolfo Lutz; Seção de Vírus Transmitidos por Artrópodos
  • Cecília L. S. Santos Instituto Adolfo Lutz; Laboratório de Biologia Molecular
  • Akemi Suzuki Instituto Adolfo Lutz; Seção de Vírus Transmitidos por Artrópodos
  • Selma M. C. Petrella Instituto Adolfo Lutz; Seção de Vírus Transmitidos por Artrópodos
  • Ivani Bisordi Instituto Adolfo Lutz; Seção de Vírus Transmitidos por Artrópodos
  • Adélia H. Nagamori Instituto Adolfo Lutz; Seção de Vírus Transmitidos por Artrópodos
  • Antonia T. Marti Instituto Adolfo Lutz; Seção de Vírus Transmitidos por Artrópodos
  • Raimundo N. Santos Instituto Adolfo Lutz; Seção de Vírus Transmitidos por Artrópodos
  • Danya M. Fialho Instituto Adolfo Lutz; Seção de Vírus Transmitidos por Artrópodos
  • Shirlene Lavigne Instituto Adolfo Lutz; Laboratório de Biologia Molecular
  • Marcia R. Buzzar Centro de Vigilância Epidemiológica; Divisão de Zoonoses
  • Iray M. Rocco Instituto Adolfo Lutz; Seção de Vírus Transmitidos por Artrópodos

Keywords:

Mayaro, Alphavirus, Virus isolation, Human infection

Abstract

Mayaro virus (MAYV) is an arbovirus (Togaviridae: Alphavirus) enzootic in tropical South America and maintained in a sylvan cycle involving wild vertebrates and Haemagogus mosquitoes. MAYV cases occur sporadically in persons with a history of recent activities inside or around forests. This paper reports three cases of MAYV fever detected in men infected in Camapuã, MS, Brazil. Serum samples collected at four days and two months after the onset of the symptoms and examined by hemagglutination inhibition test, revealed monotypic seroconversion to MAYV. Isolation of the virus was obtained from one of the samples by inoculation of the first blood samples into newborn mice. A suspension of the infected mouse brain was inoculated into C6/36 cells culture and the virus was identified by indirect immunofluorescent assay with alphavirus polyclonal antibodies. RT-PCR, performed with RNA extracted from the supernatant of C6/36 infected cells in the presence of alphavirus generic primers as well as specific MAYV primers, confirmed these results. The reported cases illustrate the importance of laboratory confirmation in establishing a correct diagnosis. Clinical symptoms are not always indicative of a disease caused by an arbovirus. Also MAYV causes febrile illness, which may be mistaken for dengue.

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Published

2007-08-01

Issue

Section

Virology

How to Cite

Coimbra, T. L. M., Santos, C. L. S., Suzuki, A., Petrella, S. M. C., Bisordi, I., Nagamori, A. H., Marti, A. T., Santos, R. N., Fialho, D. M., Lavigne, S., Buzzar, M. R., & Rocco, I. M. (2007). Mayaro virus: imported cases of human infection in São Paulo State, Brazil . Revista Do Instituto De Medicina Tropical De São Paulo, 49(4), 221-224. https://revistas.usp.br/rimtsp/article/view/31097