Development and validation of equipment for kinematic and sensory-motor analysis of experimental spinal injury induced in rats
DOI:
https://doi.org/10.11606/issn.2176-7262.rmrp.2025.221597Palabras clave:
Spinal cord injury, kinematic analysis, Sensorimotor analysis sciatic, Functional indexResumen
Spinal cord injury (SCI) is defined as an injury to the spinal cord, a structure responsible for regulating numerous functions in the body. Depending on the level and degree of the SCI, a rapid and devastating loss of neurological functions of anatomical structures located below the level of the lesion is observed. The objective of this study was to develop and validate equipment for kinematic and sensorimotor analysis of rats with induced spinal cord injury. A glass box was manufactured in the shape of a linear corridor through which the animals moved. A transparent acrylic plate was positioned inside the box, fixed by nylon cables to a load cell. The system works as a suspended platform to capture the force vectors applied by the rats during their movement. The box was supported on two wooden supports on a table, and between the two supports below the glass box, a mirror was positioned at 45º in order to display the bottom of the glass box. A camera was positioned in front of the mirror 50cm away to capture images of the rats' paws with the aim of enabling measurements of the variables used in calculating the sciatic functional index. The data was exported and post-processed to obtain graphical, quantitative and comparative analysis of the results. To validate the equipment, a statistical measurement correlation model was used, and in addition, clinical trials were carried out verifying the average speed, load distribution force and the sciatic functional index of the rats. The equipment was capable of fully collecting the proposed data during the passage through the platform, as well as capturing images of the paws through the mirror and storing the information in logs for post-processing of the data. As a result of the interclass correlation coefficient (ICC), the equipment achieved indices of 0.967 for ground reaction force; 0.896 for the sciatic functional index (SFI) and 0.755 for average speed. The fact that there is, in single piece of equipment, a way to capture the ground reaction force digitally and in real time allowed the storage of this information in .log format files, which made it possible to process and interpret it in graphic form and tabulated, that is, a reliable and accurate way of representing the behavior of individuals while being evaluated. It is concluded that the equipment developed was capable of capturing and storing data relating to kinematic and sensorimotor parameters.
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Derechos de autor 2026 Luis Filipe Karaanasov Beloni, Alessandro Corrêa Mendes, Emília Ângela Lo Schiavo Arisawa, Fernanda Pupio Silva Lima, Luciana Barros Sant’Anna, Mário Oliveira Lima
Esta obra está bajo una licencia internacional Creative Commons Atribución 4.0.
