Genetic divergence among hybrids of 'Cravo' mandarin with 'Pêra' sweet orange

Authors

  • Roberto Pedroso de Oliveira Embrapa Clima Temperado
  • Carlos Ivan Aguilar-Vildoso Centro de Citricultura Sylvio Moreira
  • Marcos Antônio Machado Centro de Citricultura Sylvio Moreira

DOI:

https://doi.org/10.1590/S0103-90162003000100017

Keywords:

Citrus reticulata, Citrus sinensis, Tocher grouping, RAPD, citrus

Abstract

Molecular markers have been used as tools in breading programs of sexual hybridation, allowing the genetic characterization of a large number of genotypes. The RADP markers are the most used since the employed techniques are simple and of low cost. To evaluate the genetic divergence among F1 hybrids of 'Cravo' mandarin (Citrus reticulata Blanco) and 'Pêra' sweet orange (C. sinensis (L.) Osbeck), this study analyses the variability and similarity of the hybrids among themselves and with their parents. Random Amplified Polimorfic DNA marker analysis, with 102 primers, were applied to a population composed of 94 hybrids and their parents. Multivariate genetic divergence analysis of the principal components and Tocher grouping were carried out only considering the polymorphic fragments. Genetic distances were calculated by the arithmetic complement of the Jaccard index. Bidimensional dispersion graphs among hybrid and parent distances and of the divergence analysis by principal components were constructed. High genetic similarity among Cravo and Pêra varieties and their hybrids was verified, showing a casual distribution from the hybrids in relation to the parents, but in intermediary positions. The principal component analysis showed little applicability in the study of hybrid genetic divergence. The hybrids and parents were classified in groups based on the genetic similarity, using the Tocher optimization method.

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Published

2003-02-01

Issue

Section

Genetics and Plant Breeding

How to Cite

Genetic divergence among hybrids of ’Cravo’ mandarin with ’Pêra’ sweet orange . (2003). Scientia Agricola, 60(1), 115-118. https://doi.org/10.1590/S0103-90162003000100017