Phytoplasma associated with shoot proliferation in begonia

Authors

  • Luiz Fernando Caldeira Ribeiro USP; ESALQ; Depto. de Entomologia, Fitopatologia e Zoologia Agrícola
  • Ana Paula de Oliveira Amaral Mello USP; ESALQ; Depto. de Entomologia, Fitopatologia e Zoologia Agrícola
  • Ivan Paulo Bedendo USP; ESALQ; Depto. de Entomologia, Fitopatologia e Zoologia Agrícola
  • Ricardo Gioria Sakata Seed Sudamérica

DOI:

https://doi.org/10.1590/S0103-90162006000500009

Keywords:

Mollicutes, yellows, phytopathogenic prokaryotes

Abstract

Begonia is a very appreciated genus of ornamental plants, of economic relevancy, having species of flowers and foliage. In commercial croppings, plants exhibiting characteristic symptoms of phytoplasma infection have been observed, such as shoot proliferation, reduced plant, size small leaves and flowers, and phyllody. Leaves were sampled and total DNA was extracted to be used in nested Polymerase Chain Reaction (PCR), in order to detect and identify an expected phytoplasma. The results confirmed consistently the presence of a phytoplasma associated with symptomatic plants through the amplification of a typical genomic fragment of 1.2 kb by using the universal primers R16mF2/mR1 and R16F2n/R2. The use of specific primers R16(III)F2/R1 allowed to identify the phytoplasma detected as a representative of the group 16SrIII. This information is very expressive, because different diseases caused by fungus, bacteria, virus and nematodes have been reported for begonia, however, reports have not been found for begonia diseases associated with phytoplasmas.

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Published

2006-10-01

Issue

Section

Genetics and Plant Breeding

How to Cite

Phytoplasma associated with shoot proliferation in begonia . (2006). Scientia Agricola, 63(5), 475-477. https://doi.org/10.1590/S0103-90162006000500009