Detection of infectious bronchitis virus and Avian metapneumovirus by a duplex semi-nested reverse transcription–polymerase chain reaction assay

Authors

  • Ana Maria Acevedo Department of Animal Virology, Microbiology Direction. National Center for Animal and Plant Health, Havana, Cuba; Coronavirus Research Group, School of Veterinary Medicine, University of São Paulo
  • Laura Yaneth B. Villarreal Coronavirus Research Group, School of Veterinary Medicine, University of São Paulo
  • Leonardo José Richtzenhain Department of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine, University of São Paulo; Coronavirus Research Group, School of Veterinary Medicine, University of São Paulo
  • Julia Noda Department of Animal Virology, Microbiology Direction. National Center for Animal and Plant Health, Havana
  • Paulo Eduardo Brandão Department of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine, University of São Paulo; Coronavirus Research Group, School of Veterinary Medicine, University of São Paulo

DOI:

https://doi.org/10.11606/issn.1678-4456.v49i3p221-224

Keywords:

IBV. aMPV. PCR. Diagnosis.

Abstract

A duplex RT-PCR assay is reported for the simultaneous detection of avian infectious bronchitis virus (IBV) and avian metapneumovirus (aMPV), the causative agents of major diseases in poultry. The duplex RT-PCR assay optimized showed a detection limit of 10-3 (101 EID50/50m L for IBV and 100.5 EID50/50m L for aMPV, respectively when two viruses were mixed and 10-1 for each one separated (103 EID50/50m L for IBV and 102.5 EID50/50m L for aMPV, respectively. It was specific, sensitive and applicable for the rapid detection of these viruses in clinical samples.

 

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Published

2012-06-06

Issue

Vol. 49 No. 3 (2012)

Section

UNDEFINIED

License

The journal content is authorized under the Creative Commons  BY-NC-SA  license (summary of the license: https://creativecommons.org/licenses/by-nc-sa/4.0 | full text of the license: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode) 

How to Cite

1.
Acevedo AM, Villarreal LYB, Richtzenhain LJ, Noda J, Brandão PE. Detection of infectious bronchitis virus and Avian metapneumovirus by a duplex semi-nested reverse transcription–polymerase chain reaction assay. Braz. J. Vet. Res. Anim. Sci. [Internet]. 2012 Jun. 6 [cited 2024 Dec. 4];49(3):221-4. Available from: https://revistas.usp.br/bjvras/article/view/42205