Minimal inhibitory concentration (MIC) determination of disinfectant and/or sterilizing agents

Authors

  • Priscila Gava Mazzola State University of Campinas; Faculty of Medical Sciences; Department of Clinical Pathology
  • Angela Faustino Jozala University of São Paulo; Faculty of Pharmaceutical Sciences; Department of Biochemical-Pharmaceutical Technology
  • Letícia Célia de Lencastre Novaes University of São Paulo; Faculty of Pharmaceutical Sciences; Department of Biochemical-Pharmaceutical Technology
  • Patricia Moriel State University of Campinas; Faculty of Medical Sciences; Department of Clinical Pathology
  • Thereza Christina Vessoni Penna University of São Paulo; Faculty of Pharmaceutical Sciences; Department of Biochemical-Pharmaceutical Technology

DOI:

https://doi.org/10.1590/S1984-82502009000200008

Keywords:

Minimal inhibitory concentration, Disinfectant agents, Sterilizing agents, Sanitation^i1^sprogr

Abstract

Due to the growing number of outbreaks of infection in hospital and nurseries, it becomes essential to set up a sanitation program that indicates that the appropriate chemical agent was chosen for application in the most effective way. Validating the effectiveness of decontamination and disinfection is an important and often challenging task. In order to study and compare the behavior of selected microorganisms, they were submitted to minimal inhibitory concentration (MIC). The MIC intervals, which reduced bacteria populations over 6 log10, were: 59 to 156 mg/L of quaternary ammonium compounds (QACs); 63 to 10000 mg/L of chlorhexidine; 1375 to 3250 mg/L of glutaraldehyde; 39 to 246 mg/L of formaldehyde; 43750 to 87500 mg/L of ethanol; 1250 to 6250 mg/L of iodine in polyvinyl-pyrolidone complexes, 150 to 4491 mg/L of chlorine-releasing-agents (CRAs) and 469 to 2500 mg/L of hydrogen peroxide. Chlorhexidine showed non inhibitory activity over germinating spores. A. calcoaceticus showed resistance to the majority of the agents tested, followed by E. cloacae and S. marcescens.

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Published

2009-06-01

Issue

Section

Original Papers

How to Cite

Minimal inhibitory concentration (MIC) determination of disinfectant and/or sterilizing agents . (2009). Brazilian Journal of Pharmaceutical Sciences, 45(2), 241-248. https://doi.org/10.1590/S1984-82502009000200008