Comparison of microbiological assay and HPLC-UV for determination of fluconazole in capsules

Authors

  • Kelly Marques Queiroz Universidade Vale do Rio Doce; Faculdade de Ciências da Saúde; Laboratório de Microbiologia
  • Maria-Luiza Martins Silva Universidade Vale do Rio Doce; Faculdade de Ciências da Saúde; Laboratório de Microbiologia
  • Nathália Duque Prado Universidade Vale do Rio Doce; Faculdade de Ciências da Saúde; Laboratório de Microbiologia
  • Paulo Marcelo Andrade Lima Universidade Vale do Rio Doce; Faculdade de Ciências da Saúde; Laboratório de Microbiologia
  • Rosiane Dias Lopes Diniz Universidade Vale do Rio Doce; Faculdade de Ciências da Saúde; Laboratório de Microbiologia
  • Isabela Costa César Universidade Federal de Minas Gerais; Faculdade de Farmácia; Departamento de Produtos Farmacêuticos
  • Gerson Antônio Pianetti Universidade Federal de Minas Gerais; Faculdade de Farmácia; Departamento de Produtos Farmacêuticos
  • Daniel Assis Santos Universidade Vale do Rio Doce; Faculdade de Ciências da Saúde; Laboratório de Microbiologia

DOI:

https://doi.org/10.1590/S1984-82502009000400012

Keywords:

Fluconazole^i1^squantitative determinat, Microbiological assay^i1^sagar diffus, High performance liquid chromatography

Abstract

The development of a specific agar diffusion bioassay for the quantitative determination of fluconazole formulated in capsules was carried out using a strain of Candida albicans ATCC 18804 as the test organism. A prospective validation of the method showed adequate linearity (r²=0.9995), precision (R.S.D. = 4.0% for intra-day and 4.5% for inter-day precision) and accuracy (mean recovery = 102.9%). High performance liquid chromatography was chosen as a comparison method for the fluconazole determination. The contents of fluconazole determined by both methods, for four capsule samples, showed a strong correlation, confirmed by Pearson's correlation coefficient value (r = 0.9884). The bioassay is a suitable method for both research and pharmaceutical industry laboratories.

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Published

2009-12-01

Issue

Vol. 45 No. 4 (2009)

Section

Original Papers

License

All content of the journal, except where identified, is licensed under a Creative Commons attribution-type BY.

The on line journal has open and free access.

How to Cite

Comparison of microbiological assay and HPLC-UV for determination of fluconazole in capsules . (2009). Brazilian Journal of Pharmaceutical Sciences, 45(4), 693-700. https://doi.org/10.1590/S1984-82502009000400012