An alternate technique for isolation of Toxocara canis excretory-secretory antigens

Authors

  • Cristiane Maria Colli State University of Maringá; Department of Clinical Analysis
  • Guita Rubinsky Elefant Institute of Tropical Medicine of São Paulo
  • Marcia Liz Paludo University of Paraná
  • Ana Lúcia Falavigna Guilherme State University of Maringá; Department of Clinical Analysis

DOI:

https://doi.org/10.1590/S1984-82502011000100015

Keywords:

Toxocara canis^i1^sexcretory-secretory antig, Antigens^i1^sisolat, Dialysis

Abstract

The aim of the present study was to test the effectiveness of a sausage-casing membrane for dialysis of Toxocara excretory-secretory antigens (TES). The protein concentrated by the tested membrane was compared with that obtained using a Sigma commercial membrane, as were the protein fractions found by polyacrylamide gel electrophoresis. Standard positive and negative serum samples were evaluated in an ELISA immunoassay, and equivalent data were obtained in all steps, indicating that the sausage-casing membrane is efficient, besides being less expensive to process.

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Published

2011-03-01

Issue

Vol. 47 No. 1 (2011)

Section

Articles

License

All content of the journal, except where identified, is licensed under a Creative Commons attribution-type BY.

The on line journal has open and free access.

How to Cite

An alternate technique for isolation of Toxocara canis excretory-secretory antigens . (2011). Brazilian Journal of Pharmaceutical Sciences, 47(1), 119-123. https://doi.org/10.1590/S1984-82502011000100015