Silver nanoparticles incorporated into nanostructured biopolymer membranes produced by electrospinning: a study of antimicrobial activity

Authors

  • Karen Segala University of Campinas; School of Chemical Engineering; Department of Materials Engineering and Bioprocess
  • Silvia Vaz Guerra Nista University of Campinas; School of Chemical Engineering; Department of Materials Engineering and Bioprocess
  • Lívia Cordi University of Campinas; Chemistry Institute
  • Maria Trindade Marques Bizarria University of Campinas; School of Chemical Engineering; Department of Materials Engineering and Bioprocess
  • José de Ávila Júnior Federal University of São João del-Rey; Mechanical Engineering Department
  • Sirlene Adriana Kleinubing University of Campinas; School of Chemical Engineering; Department of Materials Engineering and Bioprocess
  • Deborah Cristina Cruz University of Campinas; School of Chemical Engineering; Department of Materials Engineering and Bioprocess
  • Marcelo Brocchi University of Campinas; Institute of Biology; Department of Genetics, Evolution and Bioagents
  • Liliane Maria Ferrareso Lona University of Campinas; School of Chemical Engineering; Department of Materials Engineering and Bioprocess
  • Nelson Eduardo Durán Caballero UNICAMP; Institute of Chemistry
  • Lucia Helena Innocentini Mei University of Campinas; School of Chemical Engineering; Department of Materials Engineering and Bioprocess

DOI:

https://doi.org/10.1590/S1984-82502015000400017

Abstract

This study examines the antimicrobial activity of silver nanoparticles incorporated into nanostructured membranes made of cellulose acetate (CA) and blends of chitosan/poly-(ethylene oxide, CTS/PEO) and prepared by electrospinning. The formation of chemically synthesized Ag nanoparticles (AgNPs) was monitored by UV-visible spectroscopy (UV-Vis) and characterized by transmission electron microscopy (TEM). The size distribution of the AgNPs was measured by dynamic light scattering (DLS), with an average size of approximately 20 nm. The presence of AgNPs on the surface of electrospun nanofibers was observed by field emission electron microscopy (FEG) and confirmed by TEM. The antimicrobial activity of AgNPs incorporated into nanostructured membranes made of CA and CTS/PEO electrospun nanofibers was evaluated in the presence of both Gram-positive bacteria, such as Staphylococcus aureus ATCC 29213 and Propionibacterium acnes ATCC 6919, and Gram-negative bacteria, such as Escherichia coli ATCC 25992 and Pseudomonas aeruginosa ATCC 17933. Microbiological results showed that the presence of AgNPs in CA and CTS/PEO nanostructured membranes has significant antimicrobial activity for the Gram-positive bacteria Escherichia coli and Propionibacterium acnes.

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Published

2015-12-01

Issue

Vol. 51 No. 4 (2015)

Section

Articles

License

All content of the journal, except where identified, is licensed under a Creative Commons attribution-type BY.

The on line journal has open and free access.

How to Cite

Silver nanoparticles incorporated into nanostructured biopolymer membranes produced by electrospinning: a study of antimicrobial activity . (2015). Brazilian Journal of Pharmaceutical Sciences, 51(4), 911-921. https://doi.org/10.1590/S1984-82502015000400017