Gastroprotective potential and mechanisms of action of Hedera nepalensis

Authors

  • Naeem Shahzad Lahore College of Pharmaceutical Science, Lahore, Pakistan
  • Irfan Anjum The University of Lahore. Faculty of Pharmacy
  • Haseeb Ahsan University of Sargodha
  • Alamgeer University of Punjab. University College of Pharmacy
  • Shahzad Khurrum Syed University of Management and Technology
  • Muhammad Naveed Mushtaq The University of Lahore. Faculty of Pharmacy https://orcid.org/0000-0003-0587-7701

DOI:

https://doi.org/10.1590/s2175-97902023e20493

Keywords:

Antioxidant, Diclofenac, Ethanol, Gastroprotective, Hedera nepalensis

Abstract

Hedera nepalensis (H. nepalensis) , belonging to the family Araliaceae, is a medicinal plant traditionally used to treat stomach problems. The current study investigated the gastroprotective potential and the mechanism of action of H. nepalensis in diclofenac-and ethanol-induced ulcer models. Anti-oxidant and lipid peroxidation inhibitory prospects of H. nepalensis were checked out by free radical scavenging assay and UV spectrophotometer respectively. Effect of H. nepalensis on the pH, gastric total acidity of gastric juice and protective effects of H. nepalensis against ulcer models have been examined. Histopathological studies have been carried out. The aqueous methanol extract of H. nepalensis (100 µg/mL) showed anti-oxidant (83.55%) and lipid peroxidation inhibitory (70.88%) potential at 1000 µg/mL; the extract had no buffer potential. The extract (400 mg/kg) significantly (81.12% and 63.46%) showed gastroprotective effect in diclofenac and ethanol-induced rat ulcer models respectively. Histopathological studies confirmed the biochemical findings. FTIR analysis showed the presence of carboxylic acid, alkanes, conjugated alkanes, aldehydes and alkyl-aryl ethers. Gallic acid, M-coumaric acid and quercetin were found by HPLC analysis. H. nepalensis exhibited significant protection against diclofenac and ethanol induced gastric damage by anti-oxidant and lipid peroxidation suppression effects suggesting potential broad utility in treatment of diseases characterized with gastric damage.

Downloads

Download data is not yet available.

References

Abebaw M, Mishra B, Gelayee DA. Evaluation of anti-ulcer activity of the leaf extract of Osyris quadripartita Decne. (Santalaceae) in rats. J Exp Pharmacol. 2017;9:1-11.

Adefisayo MA, Akomolafe RO, Akinsomisoye SO, Alabi QK, Ogundipe OL, Omole JG, et al. Gastro-protective effect of methanol extract of Vernonia amygdalina (del.) leaf on aspirin-induced gastric ulcer in Wistar rats.” Toxicol Rep. 2017;4:625-633.

Ahmad SS, Javed S. Exploring the economic value of underutilized plant species in Ayubia National Park. Pak J Bot. 2007;39(5):1435-1442.

Akhtar N, Rashid A, Murad W, Bergmeier EJ. Diversity and use of ethno-medicinal plants in the region of Swat, North Pakistan. J Ethnobiol Ethnomed. 2013;9:25.

Awan AF, MS Akhtar, I Anjum, Mushtaq MN, Fatima A, Khan MUR, et al. Hepatoprotective effect of Ziziphus oxyphylla Edgew in paracetamol-induced hepatotoxic rat model. Pak J Pharm Sci. 2020;33(5):2449-2454.

Chandra S. Fourier transform infrared (Ft-Ir) spectroscopic analysis of Nicotiana plumbaginifolia (Solanaceae). J Med Plants Studies. 2019;7(1):82-85.

Chung KT, Shelat VG. Perforated peptic ulcer - an update. World J Gastrointest Surg. 2017;9(1):1-12. doi: 10.4240/wjgs.v9.i1.1. PMID: 28138363; PMCID: PMC5237817.

» https://doi.org/10.4240/wjgs.v9.i1.1

De Barros MP, Lemos M, Maistro EL, Leite MF, Sousa JPB, Bastos JK, et al. Evaluation of antiulcer activity of the main phenolic acids found in brazilian green propolis. J Ethnopharmacol. 2008;120(3):372-377.

El-Hady FKA, El Awdan SA, Ibrahim AM. Anti-ulcerative potential of Egyptian propolis against oxidative gastric injury induced by indomethacin in rats. Adv J Med Plant Res. 2013;3(2):35-42.

Hashmi WJ, Ismail H, Mehmood F, Mirza B. Neuroprotective, antidiabetic and antioxidant effect of Hedera nepalensis and lupeol against STZ+ AlCl 3 induced rats model. DARU. 2018;26(2):179-190.

Heinonen IM, Lehtonen PJ, Hopia AI. Antioxidant activity of berry and fruit wines and liquors. J Agric Food Chem. 1998;46(1):25-31.

Ismail IF, Golbabapour S, Hassandarvish P, Hajrezaie M, Abdul Majid N, Kadir FA, et al. Gastroprotective activity of Polygonum chinense aqueous leaf extract on ethanol-induced hemorrhagic mucosal lesions in rats. Evid Based Complementary Altern Med. 2012;404012. doi: 10.1155/2012/404012.

» https://doi.org/10.1155/2012/404012

Jahantighi A, G Kiani, TT Moghaddam and S Ghahari. In vitro antibacterial activity of selected medicinal plants traditionally used in Iran against plant and human pathogenic bacteria. Jordan J Bio Sci. 2016;9(3):221-226.

Jafri L, Saleem S, Ullah N, Mirza B. In vitro assessment of antioxidant potential and determination of polyphenolic compounds of Hedera nepalensis K. Koch. Arab J Chem. 2017;10(Suppl 2):S3699-S3706.

Jennings A, Welch AA, Fairweather-Tait SJ, Kay C, Minihane A-M, Chowienczyk P, et al. Higher anthocyanin intake is associated with lower arterial stiffness and central blood pressure in women. Am J Clin Nutr. 2012;96(4):781-788.

Kang J-W, Yun N, Han H-J, Kim J-Y, Kim J-Y, Lee S-M. Protective effect of flos lonicerae against experimental gastric ulcers in rats: mechanisms of antioxidant and anti-inflammatory action. Evid Based Complementary Altern Med . 2014;596920. doi: 10.1155/2014/596920

» https://doi.org/10.1155/2014/596920

Kanwal S, Ullah N, Haq I, Afzal I, Mirza B. Antioxidant, antitumor activities and phytochemical investigation of Hedera nepalensis K. Koch, an important medicinal plant from Pakistan. Pak J Bot. 2011;43:85-89.

Kim S-H, Jun C-D, Suk K, Choi B-J, Lim H, Park S, et al. Gallic acid inhibits histamine release and pro-inflammatory cytokine production in mast cells. Toxicol Sci. 2006;91(1):123-131.

Klein LC, Gandolfi RB, Santin JR, Lemos M, Cechinel Filho V, de Andrade SF. Antiulcerogenic activity of extract, fractions, and some compounds obtained from Polygala cyparissias St. Hillaire & Moquin (Polygalaceae). Naunyn Schmiedbergs Arch Pharmacol. 2010;381(2):121-126.

Lee M, Kallal S, Feldman M. Omeprazole prevents indomethacin-induced gastric ulcers in rabbits. Aliment Pharmacol Ther. 1996;10(4):571-576.

Liu X, Zakaria M, Islam M, Radhakrishnan R, Ismail A, Chen H, et al. Anti-inflammatory and anti-ulcer activity of Calligonum comosum in rats. Fitotherapia. 2001;72(5):487-491.

Luna JS, Dos Santos A, De Lima M, De Omena M, De Mendonça F, Bieber L, et al. A study of the larvicidal and molluscicidal activities of some medicinal plants from northeast Brazil. J Ethnopharmacol. 2005;97(2):199-206.

Martin M, La-Casa C, Alarcon-de-La-Lastra C, Cabeza J, Villegas I, Motilva V. Anti-oxidant mechanisms involved in gastroprotective effects of quercetin. Z Naturforsch C J Biosci. 1998;53(1-2):82-88.

Muthusamy V, Anand S, Sangeetha K, Sujatha S, Arun B, Lakshmi B. Tannins present in Cichorium intybus enhance glucose uptake and inhibit adipogenesis in 3T3-L1 adipocytes through PTP1B inhibition. Chem Biol Interact. 2008;174(1):69-78.

Sannomiya M, Fonseca VB, Da Silva MA, Rocha LRM, Santos LC dos, Hiruma-Lima CA, et al. Flavonoids and antiulcerogenic activity from Byrsonima crassa leaves extracts. J Ethnopharmacol. 2005;97(1):1-6, 2005.

Odeja O, Obi G, Ogwuche CE, Elemike EE, Oderinlo Y. Retracted Article: Phytochemical Screening, Antioxidant and Antimicrobial activities of Senna occidentalis (L.) leaves Extract. Clin Phytosci. 2015;1(6):1-6.

Pratt D. Natural antioxidants from plant material. ACS Publications. 1992. Chapter 5; 54-7. DOI: 10.1021/bk-1992-0507.ch005

» https://doi.org/10.1021/bk-1992-0507.ch005

Qureshi RA, Ghufran MA, Gilani SA, Sultana K, Ashraf M. Ethnobotanical studies of selected medicinal plants of Sudhan Gali and Ganga Chotti Hills, District Bagh, Azad Kashmir. Pak J Bot. 2007;39(7):2275-2283.

Ramis IB, Moraes EPd, Fernandes MS, Mendoza-Sassi R, Rodrigues O, Juliano CRV, et al. Evaluation of diagnostic methods for the detection of helicobacter pylori in gastric biopsy specimens of dyspeptic patients. Braz J Microbiol. 2012;43(3):903-908.

Sattar A, Abdo A, Mushtaq MN, Anjum I, Anjum A. Evaluation of Gastro-protective activity of Myristica fragrans on Ethanol-induced ulcer in albino rats. An Acad Bras Ciênc. 2019;91(2):e20181044. https://doi.org/10.1590/0001-3765201920181044 .

» https://doi.org/10.1590/0001-3765201920181044

Scalbert A, Manach C, Morand C, Rémésy C, Jiménez L. Dietary polyphenols and the prevention of diseases. Cri Rev Sci Nutri. 2005;45(4):287-306.

Sumbul S, Ahmad MA, Mohd A, Mohd A. Role of phenolic compounds in peptic ulcer: An overview. J Pharm BioALLied Sci. 2011;3(3):361-367.

Tiong SH, Looi CY, Hazni H, Arya A, Paydar M, Wong WF, et al. Antidiabetic and antioxidant properties of alkaloids from Catharanthus roseus (L.) G. Don. Molecules. 2013;18(8):9770-9784.

Tokuşoğlu Ö, Ünal M, Yildirim Z. HPLC-UV and GC-MS characterization of the flavonol aglycons quercetin, kaempferol, and myricetin in tomato pastes and other tomato-based products. Acta Chromatogr. 2003;13(13):196-207.

Wallace JL. Prostaglandins, NSAIDs, and gastric mucosal protection: why doesn’t the stomach digest itself? Physiol Rev. 2008;88(4):1547-1565.

Yamamoto K, Kakegawa H, Ueda H, Mutsumoto H, Sudo T, Miki T, et al. Gastric cytoprotective anti-ulcerogenic actions of hydroxychalcones in rats. Planta Med. 1992;58(5):389-393.

Downloads

Published

2023-06-29

Issue

Section

Original Article

How to Cite

Gastroprotective potential and mechanisms of action of Hedera nepalensis. (2023). Brazilian Journal of Pharmaceutical Sciences, 59, e20493. https://doi.org/10.1590/s2175-97902023e20493