Development and validation of an HPLC method for the determination of fluorouracil in polymeric nanoparticles
DOI:
https://doi.org/10.1590/S1984-82502013000100013Keywords:
Fluorouracil^i1^sdeterminat, Nanoparticles, High performance liquid chromatography^i1^squantitative analy, High performance liquid chromatography^i1^smethod validatAbstract
The objective of this work was to develop and validate a rapid high performance liquid chromatography (HPLC) method for the quantitative analysis of fluorouracil (5-FU) in polymeric nanoparticles. Chromatographic analyses were performed on an RP C18 column with a mobile phase consisting of acetonitrile and water (10:90, v/v) at a flow rate of 1 mL/min. The 5-FU was detected and quantitated using a photodiode array detector at a wavelength of 265 nm. The method was shown to be specific and linear in the range of 0.1-10 µg/mL (r = 0.9997). The precision (intra- and inter-day) was demonstrated because the maximum relative standard deviation was 3.51%. The method is robust relative to changes in flow rate, column and temperature. The limits of detection and quantitation were 10.86 and 32.78 ng/mL, respectively. The method fulfilled the requirements for reliability and feasibility for application to the quantitative analysis of 5-FU in polymeric nanoparticles.Downloads
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Published
2013-03-01
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Development and validation of an HPLC method for the determination of fluorouracil in polymeric nanoparticles . (2013). Brazilian Journal of Pharmaceutical Sciences, 49(1), 117-126. https://doi.org/10.1590/S1984-82502013000100013