Alkaloid-rich fraction of Himatanthus lancifolius contains anti-tumor agents against leukemic cells

Authors

  • Melissa Pires de Lima Federal University of Paraná; Department of Medical Pathology; Laboratory of Hematology
  • Luciana Farhat Hilst Federal University of Paraná; Department of Medical Pathology; Laboratory of Hematology
  • Fernanda Vanessa Rechinbach Mattana Federal University of Paraná; Department of Medical Pathology; Laboratory of Hematology
  • Cid Aimbiré de Moraes Santos Federal University of Paraná; Department of Pharmacy; Laboratory of Pharmacognosy
  • Almeriane Maria Weffort-Santos Federal University of Paraná; Department of Pharmacy; Laboratory of Pharmacognosy

DOI:

https://doi.org/10.1590/S1984-82502010000200014

Keywords:

Apocynaceae, Cell proliferation, Himatanthus lancifolius, Indole alkaloids, Leukemia, Plant extract

Abstract

The effects of the alkaloid-rich fraction of Himatanthus lancifolius (Müll. Arg) Woodson on normal marrow cells and leukemic cell lines were investigated. After 48 h exposure, the proliferation assay showed significant cell growth inhibition for Daudi (0.1-10 µg/mL), K-562 (1-10 µg/mL), and REH cells (10-100 µg/mL), yet was inert for normal marrow cells. A similar inhibition profile was observed in clonogenic assays. This alkaloid-rich fraction, in which uleine is the main compound, showed no signs of toxicity to any cells up to 10 µg/mL. Cell feature analyses after induction of differentiation showed maintenance of the initial phenotype. Flow cytometric expression of Annexin-V and 7-AAD in K-562 and Daudi cells has indicated that the cells were not undergoing apoptosis or necrosis, suggesting cytostatic activity for tumor cells

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Published

2010-06-01

Issue

Vol. 46 No. 2 (2010)

Section

Original Papers

License

All content of the journal, except where identified, is licensed under a Creative Commons attribution-type BY.

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How to Cite

Alkaloid-rich fraction of Himatanthus lancifolius contains anti-tumor agents against leukemic cells . (2010). Brazilian Journal of Pharmaceutical Sciences, 46(2), 273-280. https://doi.org/10.1590/S1984-82502010000200014