Diethylcarbamazine (dec) protects against acute hepatotoxicity induced by carbon tetrachloride (ccl4) in mice by modulating pro-inflammatory markers through autophagy activation
DOI:
https://doi.org/10.1590/s2175-97902025e23935Keywords:
Diethylcarbamazine, Hepatoprotective, Anti-inflammatory, Carbon tetrachloride, AutophagyAbstract
The current study examined the effects of Diethylcarbamazine (DEC) on CCl4-induced liver toxicity and the autophagic process in mice. A dose of 50mg/kg of DEC was intragastrically administered for 12 days before the CCl4 (10%,v/v in olive oil, 2ml/kg) was administered via injection. Mice were euthanized 24h after the CCl4 injection. The rise in serum levels of NO and activity of glutathione reductase in CCl4-intoxicated mice were suppressed by DEC. Liver histopathology showed that DEC reduced the incidence of liver lesions induced by CCl4. In addition, DEC reduced the expression of TNF-α,IL-1β,IL-6,IL-22,NF-κB, MMP-9, COX-2 and iNOS in CCl4-treated mice. There was also a reduction in the expression of AKT and apoptotic cells. The expression of IL-10, IFN-γ, LC3 and PI3K increased in the DEC pre-treated group. The hepatoprotective mechanisms of DEC may be related to the attenuation of oxidative stress and anti-inflammatory activity. Thus, the impact of DEC on inflammatory and autophagic mechanisms becomes apparent, underscoring the importance of analyzing this drug's ability to induce improvements in acute toxin-induced hepatitis. Therefore, the conduct of this study is justified, as the development of new treatments and access to healthcare are paramount for the care of patients with hepatitis.
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