Activities, network pharmacology, potential mechanism against inflammation of a homogeneous polysaccharide MCP-1 from Mesona chinensis Benth

Chushan Chen; Haoming Chen; Juntao Xie; Yi Chen; Xin Yu; Hongxia Fan; Jiajin Huang; Huiqin Lu; Junxia Zheng

doi:10.1590/s2175-97902026e24378

Authors

Chushan Chen College of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou, Guangdong, China , Guangdong University of Technology
Haoming Chen College of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou, Guangdong, China , Guangdong University of Technology
Juntao Xie College of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou, Guangdong, China , Guangdong University of Technology
Yi Chen College of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou, Guangdong, China , Guangdong University of Technology
Xin Yu College of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou, Guangdong, China , Guangdong University of Technology
Hongxia Fan College of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou, Guangdong, China , Guangdong University of Technology
Jiajin Huang College of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou, Guangdong, China , Guangdong University of Technology
Huiqin Lu Guangdong Second Provincial General Hospital, Guangzhou, Guangdong, China
Junxia Zheng College of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou, Guangdong, China , Guangdong University of Technology

DOI:

https://doi.org/10.1590/s2175-97902026e24378

Keywords:

Mesona chinensis Benth, Polysaccharide, Anti-inflammatory activity, Network pharmacology, Molecular docking

Abstract

Inflammation is a condition in which the body’s immune system is compromised. Polysaccharides from Mesona chinensis Benth have been shown to repair the immune system and inhibit inflammation. This study aimed to investigate the activities, network pharmacology, and potential mechanisms of action against inflammation of a homogeneous polysaccharide, MCP-1, from M. chinensis. MCP-1 was isolated and purified using water extraction, alcohol precipitation, the Sevag method, and column chromatography. The molecular weight of MCP-1 was determined to be 70.9kDa. The polysaccharide composition included glucose (39.05%), arabinose (18.31%), galactose (14.80%), galacturonic acid (13.98%), xylose (7.42%), mannose (4.14%), and glucuronic acid (2.30%). MCP-1 showed strong inhibitory effects on the release of NO, IL-6, and TNF-α (30.79 ± 4.13%, 30.51 ± 3.25%, and 37.25 ± 3.57%, respectively). A total of 169 targets were identified based on the monosaccharide structures of MCP-1, with 9 targets associated with inflammation, including TLR4, STAT3, MMP9, COL18A1, TLR9, ABCB1, PPARA, MMP2, and NR1H4. The principal targets for anti-inflammatory therapies were associated with various signaling pathways involved in cancer and immune responses. Molecular docking experiments revealed that MCP-1 exhibited a high binding affinity for MMP2.

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Activities, network pharmacology, potential mechanism against inflammation of a homogeneous polysaccharide MCP-1 from Mesona chinensis Benth

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