Metabolic and antioxidant parameters in the kidneys of rats treated with copaiba oil in cirrhosis induced by thioacetamide
DOI:
https://doi.org/10.1590/s2175-97902026e24458Keywords:
Hepatorenal syndrome, Nephrotoxicity, Oleoresin, Oxidative stress, ThioacetamideAbstract
This study evaluated the effects of copaiba oil on the kidneys of rats with cirrhosis induced by thioacetamide. Male Wistar rats were divided into four groups (n = 8), namely Control (C), Thioacetamide (TAA), Copaiba Oil (OC), and Thioacetamide + Copaiba Oil (TAA+OC). The biochemical and immunological analyses were performed, and results for the antioxidant enzymatic analysis revealed an increase in GST activity for TAA and TAA+OC compared to C. For GSH, higher values were observed in the TAA and TAA+OC groups compared to the C, but the latter differed from OC, such as TBARS. For Vit C, the TAA+OC group differed from the C and TAA and for glucose and lactate, lower values were observed for the TAA+OC group compared to the OC group. Cytokine concentrations showed the same profile with the TAA+OC group showing lower values compared to the C and OC groups. The data obtained do not show a clear positive effect of copaiba oil on kidney tissue when compared to the potential damage caused by thioacetamide. However, the groups that received either thioacetamide or copaiba oil alone maintained their basal levels, indicating that, at the doses used, these compounds did not negatively alter renal morphology.
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Alqrad MAI, El-Agamy DS, Ibrahim SEM, Sirwi A, Abdallah HM, Abdel-Sattar E, et al. SIRT1/Nrf2/NF-B Signaling Mediates Anti-Inflammatory and Anti-Apoptotic Activities of Oleanolic Acid in a Mouse Model of Acute Hepatorenal Damage. Medicina. 2023;59:1351. https://doi.org/10.3390/medicina59071351
Alshahrani MY, Ebrahim HA, Alqahtani SM, Bayoumy NM, Kamar SS, ShamsEldeen AM, et al. Metformin Suppresses Thioacetamide-Induced Chronic Kidney Disease in Association with the Upregulation of AMPK and Downregulation of Oxidative Stress and Inflammation as well as Dyslipidemia and Hypertension. Molecules. 2023;28:2756. DOI:10.3390/molecules28062756
Ames-Sibin AP, Barizão CL, Castro-Ghizoni CV, Silva FMS, Sá-Nakanishi AB, Bracht L, et al. β-Caryophyllene, the major constituent of copaiba oil, reduces systemic inflammation and oxidative stress in arthritic rats. J Cell Biochem. 2018;119(12):10262-10277. DOI: 10.1002/jcb.27369.
Bradford MMA. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72:248-254. DOI: 10.1016/0003-2697(76)90527-3.
Brown FR, Mcandams AJ, Cummins JW, Konkol R, Singh I, Moser HW. Cerebro-hepato-renal (Zellweger) syndrome and neonatal adrenoleukodystrophy: Similarities in phenotype and accumulation of very long chain fatty acids. Johns Hopkins Med J 1982;151(6):344-351. PMID: 7176294.
Buege JA, Aust SD. Microsomal lipid peroxidation. Methods Enzymol. 1978;52:302-310. DOI: 10.1016/S0076-6879(78)52032-6.
Cartaxo YA, Junior FCO, Silva ML, Feitosa ANA. Diagnóstico e tratamento da síndrome hepatorrenal: Uma Revisão Integrativa. Rev Interdisc Saúde. 2018;5(4):724-742. ISSN: 2358-7490.
Colombo G, Clerici M, Garavaglia ME, Giustarini D, Rossi R, Milzani A, Dalle-Donne I. A step-by-step protocol for assaying protein carbonylation in biological samples. J Chromatogr B Analyt Technol Biomed Life Sci. 2016;1019:178–190. DOI:10.1016/j.jchromb.2015.11.052
Da Silva KC, Aguiar DH, Bomfim GF. Assessment of the effect of copaiba oil (copaifera sp.) on the kidneys of rats with hepatic dysfunction. Sci Electron Arch. 2023;16(5). DOI:10.36560/16520231722.
De Paula M, Rocha LA, Telles LO, Mendonça ST, Sinhorin VDG, Nascimento AF, et al. Copaiba oleoresin attenuates weight gain without alter inflammatory and redox system markers in adipose tissue of healthy animals. Sci Elec Arch. 2023;16(4):53-59. DOI: 10.36560/16420231694.
Duboie M, Gilles KA, Hamilton JK, Rebers PT, Smith F. Colorimetric method for determination of sugars and related substances. Anal Biochem. 1956;28(3):350-358. DOI: 10.1021/ac60111a017. DOI: 10.1111/j.1742-7843.2012.00903.x.
Fernandes ES, Passos GF, Medeiros R, Cunha FM, Ferreira J, Campos MM, et al. Anti-inflammatory effects of compounds alpha-humulene and (-) -trans-caryophyllene isolated from the essential oil of Cordia verbenacea. Euro J Pharmacol. 2007;569:228-236. DOI: 10.1016/j.ejphar.2007.04.059.
Furtado KS, Prado MG, Silva MA, Dias MC, Rivelle DP, Rodrigues MAM, Barbisan LF. Coffee and Caffeine Protect against Liver Injury Induced by Thioacetamide in Male Wistar Rats. Basic Clinic Pharmacol Toxicol. 2012;111:339–347. DOI: 10.1111/j.1742-7843.2012.00903.x.
Gentzkow CJ, Masen JM. An accurate method for the determination of blood urea nitrogen by direct nesslerization. J Biol Chem. 1942;143:531-544. DOI: 10.1016/S0021-9258(18)72642-2. DOI: 10.1016/S0021-9258(18)72642-2.
Gonçalves ES, Silva JR, Gomes CL, Nery MBL, Navarro DMAF, Santos GKN, et al. Effects of the oral treatment with Copaifera multijuga oil on reproductive performance of male Wistar rats. Rev Bras Farmacogn. 2014;24:355-362. DOI: 10.3390/plants12051054.
Habig WH, Pabst MJ, Jacoby WB. Glutathione S-transferase, the first enzymatic step in mercapturic acid formation. J Biol Chem. 1974;249:7130-7139. DOI: 10.1016/S0021-9258(19)42083-8.
Harrower JR, Brown CH. Blood lactic acid. A micromethod adapted to field collection of microliter samples. J Appl Physiol. 1972;32:709-711. DOI: 10.1152/jappl.1972.32.5.709.
Horácio BO, Geron VL, Fávero MT, Speretta G, Menezes MF. Anti-inflamatory action of copaíba oil: possible contribution in the treatment of Metabolic Syndrome. Rev Cientif FAEMA. 2017.8(1):144-160.
Hsu DZ, Chu PY, Li YH, Chandrasekaran VRM, Liu MY. Role of flavin-containing-monooxygenase-dependent neutrophil activation in thioacetamide-induced hepatic inflammation in rats. Toxicology. 2012;298:52–58.
Islam ASMS, Mamun AA, Mahtab MA, Rahman S. Management of nutrition status in liver cirrhosis patients. BEACON Med J. 2019;2(2):32-37. ISSN: 2616-6178.
Iwakiri Y. The Systemic and Splanchnic Circulation. In: Ginès P, Kamath P, Arroyo V. Chronic Liver Failure: Mechanisms and Management. Humana Press. 2011.
Jones DP. Redefining Oxidative Stress. Antioxid Redox Signal. 2006.8(9):1865–1879. DOI: 10.1089/ars.2006.8.1865.
Kurtoğlu EL, Kayhan B, Gül M, Kayhan B, Akdoğan Kayhan M, Karaca ZM, et al. A bioactive product lipoxin A4 attenuates liver fibrosis in an experimental model by regulating immune response and modulating the expression of regeneration genes. Turk J Gastroenterol. 2019 Aug;30(8):745-757. doi: 10.5152/tjg.2019.18276.
Liguori L, Russo G, Curcio F, Bulli G, Aran L, Della-Morte D, et al. Oxidative stress, aging, and diseases. Clin Interventions in Aging. 2018;13:757-772. DOI: 10.2147/CIA.S158513.
Luca LG, Matos SP, Kreutz T, Teixeira HF, Veiga Jr VF, Araújo BV, et al. Antiinflammatory effect from a hydrogel containing nanoemulsified copaiba oil (Copaifera multijuga Hayne). AAPS Pharm Sci Tech. 2018;19:522-530. DOI: 10.1208/s12249-017-0862-6
Maciel MA, Pinto AC, Veiga Jr VF, Grynberg NF, Echevarria A. Plantas medicinais: a necessidade de estudos multidisciplinares. Quím Nova. 2002;25(3):429-38. DOI: 10.1590/S0100-40422002000300016.
Misra HP, Fridovich I. The role of superoxide anion in the auto-oxidation o epinephrine and a simple assay for superoxide dismutase. J Biol Chem. 1972;247:3170–3175. PMID: 4623845.
Nelson DL, Cox MM. Princípios de bioquímica de Lehninger. Porto Alegre: Artmed, 2014.
Nelson DP, Kiesow LA. Enthalpy of decomposition of hydrogen peroxide by catalase at 25°C (with molar extinction coefficients of H2O2 solution in the UV). Anal Biochem. 1972;49:474–478. DOI: 10.1016/0003-2697(72)90451-4.
Nguyen T, Nioi P, Pickett CB. The Nrf2-Antioxidant Response Element Signaling Pathway and Its Activation by Oxidative Stress. J Biol Chem. 2009;284:13291–13295.
Oliveira ECP, Lameira AO, Zoghbi MGB. Identificação da época de coleta do óleo-resina de copaíba (Copaifera spp.) no município de Moju, PA. Rev Bras Plant Med. 2006;8(3):14-23.
Palodetto B, Postal M, Grinoli CRE, Sartorato EL, Oliveira CA. Influence Of Glutathione S-Transferase on the Ototoxicity Caused by Aminoglycosides. Braz J Otorhinolaryngol. 2010;76(3):306-309. DOI: 10.1590/S1808-86942010000300006.
Roe JH. Methods of Biochemical Analysis. Glick D (ed). New York: Interscience Publishers. 1954.
Ramos MFS. Desenvolvimento de microcápsulas contendo a fração volátil de copaíba por spray-drying: estudo de estabilidade e avaliação farmacológica. [Tese de Doutorado]. São Paulo: Universidade de São Paulo (USP). 2006.
Saad HM, Oda SS, Sedeek EK. Protective effect of Lactéol® forte against thioacetamide-induced hepatic injury in male albino rats. Alex J Vet Sci. 2020;67:92–98.
Schoonbroodt S, Piette J. Oxidative stress interference with the nuclear factor- κB activation pathways. Biochem Pharmacol. 2000;60(8):1075-1083. DOI: 10.1016/S0006-2952(00)00371-3.
Sedlack J, Lindsay RH. Estimation of total, protein bound, and nonprotein sulphydryl groups in tissue with Ellman's reagent. Anal Biochem. 1968;25:192-205. DOI: 10.1016/0003-2697(68)90092-4.
Sharma B, John S. Hepatic Cirrhosis. In: Stat Pearls (Internet). Treasure Island (FL): StatPearls Publishing. 2021.
Silva BS, Paulino AMB, Taffarel M, Borba IG, Telles LO, Lima VV, et al. High sucrose diet attenuates oxidative stress, inflammation, and liver injury in thioacetamide-induced liver cirrhosis. Life Sci. 2021;267:118944. DIO: 10.1016/j.lfs.2020.118944.
Silvestre PM. Avaliação do selante de fibrina como arcabouço para células tronco em fígados cirróticos. [Dissertação de Mestrado]. São Paulo: Universidade Estadual Paulista (UNESP). 2015.
Simonetto DA, Gines P, Kamath PS. Hepatorenal syndrome: pathophysiology, diagnosis, and management. Br Med J. 2020;370:2687. DOI: 10.1136/bmj.m2687.
Soares ER, Monteiro EB, Silva RC, Batista A, Sobreira F, Mattos T, et al. Compostos biativos em alimentos, estresse oxidativo e inflamação: uma visão molecular da nutrição. Revista HUPE. 2015;14(3):64-72. DOI: 10.12957/rhupe.2015.19942.
Spies JR. Colorimetric procedures for amino acids. Met Enzymol. 1957;3:467–477. DOI: 10.1016/S0076-6879(57)03417-5.
Taffarel M, Silva BSD, Paulino AMB, Telles LO, Mendonça ST, Santos CVD, et al. Copaiba Oleoresin Improves Weight Gain and IL-10 Concentration, with No Impact on Hepatic Histology, in Liver Cirrhosis. Biology (Basel). 2024 Oct 23;13(11):853. DOI: 10.3390/biology13110853.
Telles LO, Silva BS, Paulino AMB, Trigueiro SM, Sinhorin VDG, Lima MCF, et al. Copaiba oleoresin presents anti-obesogenic effect and mitigates inflammation and redox imbalance in adipose tissue. Acta Amazonica. 2022;52(4):331-338. DOI: 10.1590/1809-4392202201411.
Túnez I, Muñoz MC, Villavicencio MA, Medina FJ, Prado EP, Espejo I, et al. Hepato-and neurotoxicity induced by thiocetamide: protective effects of melatonin and dimethulsulfoxide. Pharm Res. 2005;52:223-228. DOI: 10.1016/j.phrs.2005.03.007.
Vásquez SPF, Mendonça MS, Noda SN. Etnobotânica de plantas medicinais em comunidades ribeirinhas do Município de Manacapuru, Amazonas, Brasil. Acta Amazon. 2014;44:457-472. DOI: 10.1590/1809-4392201400423.
Veiga Jr VF, Pinto AC. O Gênero Copaifera L. Quím Nova. 2002;25(2):273-286. DOI: 10.1590/S0100-40422002000200016.
Wu CC, Yeung LK, Tsai WS, Tseng CF, Chu P, Huang TY, et al. Incidence and factors predictive of acute renal failure in patients with advanced liver cirrhosis. Clin Nephrol. 2006;65:28-33. DOI: 10.5414/cnp65028.
Zelko IN, Mariani TJ, Folz RJ. Superoxide dismutase multigene family: A comparison of the CuZn-SOD (SOD1), Mn-SOD (SOD2), and EC-SOD (SOD3) gene structures, evolution, and expression. Free Radic Biol Med. 2002;33(3):337-349. DOI: 10.1016/s0891-5849(02)00905-x.
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Fundação de Amparo à Pesquisa do Estado de Mato Grosso
Grant numbers Process - 571424/2014
