Antimicrobial resistance of Shiga toxin‐producing Escherichia coli isolated from sheep

Emanuelle Azambuja  Lima; Ângela Vitalina Barbosa de Assis  Silveira; Lucas  Zaiden; Angélica Franco de  Oliveira; Eric Mateus Nascimento de  Paula; Vera Lúcia Dias da  Silva; Cleusely Matias de  Souza; Elaine Cristina  Castelhano; Ariel Eurides Stella

doi:10.11606/issn.1678-4456.bjvras.2022.196807

Authors

Emanuelle Azambuja Lima Universidade Federal de Jataí, Curso de Medicina Veterinária https://orcid.org/0000-0003-4896-2711
Ângela Vitalina Barbosa de Assis Silveira Universidade Federal de Jataí, Curso de Medicina Veterinária https://orcid.org/0000-0001-5400-1197
Lucas Zaiden Universidade Federal de Jataí, Curso de Medicina Veterinária https://orcid.org/0000-0003-3705-0063
Angélica Franco de Oliveira Universidade Federal de Jataí, Curso de Medicina Veterinária https://orcid.org/0000-0002-7636-1457
Eric Mateus Nascimento de Paula Centro Universitário de Mineiros https://orcid.org/0000-0002-5948-1860
Vera Lúcia Dias da Silva Universidade Federal de Jataí, Curso de Medicina Veterinária https://orcid.org/0000-0001-6964-3643
Cleusely Matias de Souza Universidade Federal de Jataí, Curso de Medicina Veterinária https://orcid.org/0000-0003-2024-0784
Elaine Cristina Castelhano Universidade Federal de Jataí, Curso de Ciências Biológicas https://orcid.org/0000-0002-1586-2645
Ariel Eurides Stella Universidade Federal de Jataí, Programa de Pós-graduação em Biociência Animal https://orcid.org/0000-0002-0435-4901

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.2022.196807

Keywords:

STEC, Antibiotic, Beta-lactamases, Zoonoses, Toxins

Abstract

The presence of Shiga toxin-producing Escherichia coli (STEC) and resistance to beta-lactams in healthy sheep represents a potential public health risk. This study aimed to characterize STEC isolates in sheep feces for toxin production and resistance to beta lactam antibiotics. In the present study, among the 40 isolates, we found a predominance of subtype Stx1 (22/40), followed by subtype Stx1 + Stx2 (11/40), while the less prevalent group was Stx2 (7/40). Also, we found phenotypical resistance to beta-lactam antibiotics in 50% (20/40) of the strains analyzed, forming two groups, one with resistant isolates and the other with non-resistant isolates. The cytotoxicity of the isolates did not vary among the groups. In addition to having this characteristic, some multiresistant isolates produced significant amounts of toxins. This leads to the conclusion that the mechanisms of antimicrobial resistance via beta lactamases are present in sheep STEC and that the cytotoxicity of those isolates is variable regarding such resistance.

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References

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Antimicrobial resistance of Shiga toxin‐producing Escherichia coli isolated from sheep

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